Adaptation of high-growth influenza H5N1 vaccine virus in Vero cells: implications for pandemic preparedness
Autor: | Tsai-Chuan Weng, Mei-Liang Huang, Min-Shi Lee, Alan Yung-Chih Hu, Yu-Shuan Chen, Wei-Zhou Yeh, Pele Chong, Yu-Fen Tseng |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2011 |
Předmět: |
Viral Diseases
viruses Adaptation Biological lcsh:Medicine medicine.disease_cause Emerging Viral Diseases Zoonoses Chlorocebus aethiops Influenza A virus lcsh:Science Antigens Viral Avian influenza A viruses Multidisciplinary Viral Vaccine Vaccination virus diseases Influenza Vaccines Medicine Infectious diseases Research Article H5N1 vaccine Influenza vaccine Molecular Sequence Data Disaster Planning Biology Microbiology H5N1 genetic structure Virus Dogs Orthomyxoviridae Infections Virology Influenza Human Vaccine Development medicine Animals Humans Pandemics Vero Cells Duck embryo vaccine Influenza A Virus H5N1 Subtype lcsh:R Immunity Viral Vaccines Influenza Influenza A virus subtype H5N1 Clinical Immunology lcsh:Q Chickens |
Zdroj: | PLoS ONE, Vol 6, Iss 10, p e24057 (2011) PLoS ONE |
ISSN: | 1932-6203 |
Popis: | Current egg-based influenza vaccine production technology can't promptly meet the global demand during an influenza pandemic as shown in the 2009 H1N1 pandemic. Moreover, its manufacturing capacity would be vulnerable during pandemics caused by highly pathogenic avian influenza viruses. Therefore, vaccine production using mammalian cell technology is becoming attractive. Current influenza H5N1 vaccine strain (NIBRG-14), a reassortant virus between A/Vietnam/1194/2004 (H5N1) virus and egg-adapted high-growth A/PR/8/1934 virus, could grow efficiently in eggs and MDCK cells but not Vero cells which is the most popular cell line for manufacturing human vaccines. After serial passages and plaque purifications of the NIBRG-14 vaccine virus in Vero cells, one high-growth virus strain (Vero-15) was generated and can grow over 10(8) TCID(50)/ml. In conclusion, one high-growth H5N1 vaccine virus was generated in Vero cells, which can be used to manufacture influenza H5N1 vaccines and prepare reassortant vaccine viruses for other influenza A subtypes. |
Databáze: | OpenAIRE |
Externí odkaz: |