Down-regulation of human cytomegalovirus UL138, a novel latency-associated determinant, by hcmv-miR-UL36
| Autor: | Rong He, Yujing Huang, Ying Qi, Yaohua Ji, Qiang Ruan, Yanping Ma, Zhengrong Sun |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
DNA Replication
Gene Expression Regulation Viral Human cytomegalovirus viruses Cytomegalovirus Down-Regulation Biology Virus Replication General Biochemistry Genetics and Molecular Biology Cell Line Viral Proteins Downregulation and upregulation Western blot microRNA medicine Humans Luciferase Gene DNA synthesis medicine.diagnostic_test HEK 293 cells virus diseases General Medicine biochemical phenomena metabolism and nutrition medicine.disease Virology Molecular biology MicroRNAs General Agricultural and Biological Sciences |
| Zdroj: | Journal of Biosciences. 38:479-485 |
| ISSN: | 0973-7138 0250-5991 |
| DOI: | 10.1007/s12038-013-9353-4 |
| Popis: | MicroRNAs (miRNAs) are small RNAs, 19-23 nucleotides in length, which regulate a variety of cellular processes. Human cytomegalovirus (HCMV) encodes only one intronic miRNA: human cytomegalovirus microRNA UL36 (hcmv-miR-UL36). In this study, we found that over-expression of hcmv-miR-UL36 resulted in a more than threefold increase in HCMV DNA synthesis at 24 h post infection. Fifteen putative targets of hcmv-miR-UL36 were identified using hybrid PCR, one being the HCMV UL138 gene that has previously been identified as a novel latency-associated determinant of HCMV infection. Down-regulation of UL138 expression by hcmv-miR-UL36 was validated using luciferase reporter assays and Western blot analysis in HEK293 cells. In the presence of hcmv-miR-UL36, we observed a 74.6 percent decrease in luciferase activity and a 46.2 percent decrease in HCMV UL138 protein expression. Our results indicate that hcmv-miR-UL36 may be a viral miRNA contributing to HCMV replication. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|