Sensing Picomolar Concentrations of RNA Using Switchable Plasmonic Chirality
| Autor: | Anton Kuzyk, Tim Liedl, Timon Funck, Francesca Nicoli |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Circular dichroism
chirality Sequence (biology) 02 engineering and technology Hepacivirus 010402 general chemistry 01 natural sciences Catalysis plasmonics chemistry.chemical_compound Molecular recognition Microscopy Electron Transmission Limit of Detection microRNA DNA origami sensing Circular Dichroism ta1182 RNA Stereoisomerism General Chemistry General Medicine DNA 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Nucleic acid Biophysics Nucleic Acid Conformation RNA Viral 0210 nano-technology |
| Zdroj: | Angewandte Chemie (International ed. in English). 57(41) |
| ISSN: | 1521-3773 |
| Popis: | Detecting small sequences of RNA in biological samples such as microRNA or viral RNA demands highly sensitive and specific methods. Here, a reconfigurable DNA origami template has been used where a chiral arrangement of gold nanorods on the structure can lead to the generation of strong circular dichroism (CD). Switching of the cross-like DNA structure is achieved by the addition of nucleic acid sequences, which arrests the structure in one of the possible chiral states by specific molecular recognition. A specific sequence can thus be detected through the resulting changes in the plasmonic CD spectrum. We show the sensitive and selective detection of a target RNA sequence from the hepatitis C virus genome. The RNA binds to a complementary sequence that is part of the lock mechanism, which leads to the formation of a defined state of the plasmonic system with a distinct optical response. With this approach, we were able to detect this specific RNA sequence at concentrations as low as 100 pm. |
| Databáze: | OpenAIRE |
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