Are macrophages involved in early myocardial reperfusion injury?
| Autor: | Geri Fratini, Lidia Ibba Manneschi, Elena Marcelli, Avio Maria Perna, Sandra Zecchi Orlandini, Chiara Nediani, Lucia Formigli |
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| Rok vydání: | 2001 |
| Předmět: |
Male
Pulmonary and Respiratory Medicine Pathology medicine.medical_specialty Neutrophils Swine Biopsy Heart Ventricles medicine.medical_treatment Ischemia Myocardial Reperfusion Injury Lesion Western blot Animals Medicine Macrophage medicine.diagnostic_test Tumor Necrosis Factor-alpha business.industry Macrophages medicine.disease Pathophysiology Microscopy Electron Cytokine Microscopy Fluorescence Neutrophil Infiltration Female Surgery Tumor necrosis factor alpha medicine.symptom Cardiology and Cardiovascular Medicine business |
| Zdroj: | The Annals of Thoracic Surgery. 71:1596-1602 |
| ISSN: | 0003-4975 |
| DOI: | 10.1016/s0003-4975(01)02400-6 |
| Popis: | Background . Neutrophils are the predominant phagocytes in the early stages of myocardial ischemia-reperfusion response and are also implicated in the development of tissue damage. This study examined the role of recruited macrophages in the evolution of this tissue injury. Methods . Farm pigs were subjected to 30 minutes of myocardial ischemia followed by 30 minutes of reperfusion. Biopsy samples were taken from the control, ischemic, and ischemic-reperfused left ventricle wall and processed for both morphologic and biochemical analyses. In situ production of tumor necrosis factor-α was evaluated by Western blot and immunofluorescence. A full hemodynamic evaluation was also performed. Results . Myocardial ischemia and early reperfusion caused marked neutrophil and macrophage tissue accumulation and tumor necrosis factor-α production by the injured tissue. Immunofluorescence studies allowed us to localize tumor necrosis factor-α predominantly in tissue-infiltrating macrophages. No depression in the global myocardial contractile function was observed, either during ischemia or after reperfusion. Conclusions . These data suggest that the newly recruited macrophages within the ischemic and early postischemic myocardium may play a role in promoting neutrophil tissue infiltration and subsequent neutrophil-induced tissue dysfunction by producing tumor necrosis factor-α. |
| Databáze: | OpenAIRE |
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