Carboxylation, the completion step in prothrombin biosynthesis
| Autor: | Jean-Marie Girardot, Robert Delaney, B. Connor Johnson |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Vitamin
Time Factors Vitamin K Biophysics Peptide Pronase Aminopeptidases Biochemistry Aminopeptidase Residue (chemistry) chemistry.chemical_compound Glutamates Animals Trypsin Carbon Radioisotopes Amino Acids Molecular Biology chemistry.chemical_classification Cell Biology Glutamic acid Carbon Dioxide Hydrogen-Ion Concentration Chromatography Ion Exchange Electrophoresis Disc Peptide Fragments Rats Amino acid Bicarbonates Kinetics chemistry Carboxylation Prothrombin Vitamin K Deficiency Warfarin |
| Zdroj: | Biochemical and Biophysical Research Communications. 59:1197-1203 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(74)90441-0 |
| Popis: | It has been found that [14C]CO2 is incorporated into prothrombin in vivo in two hours. The amount of incorporation is increased 3 to 4 fold by the administration of vitamin K1 to the warfarin-treated vitamin K-deficient rat, over incorporation in the “normal” rat. The radioactivity is found in one acidic peptide following trypsin digestion and following pronase and aminopeptidase digestion is found in one acidic amino acid. The [14C] is lost on heating of this amino acid at pH 2, leaving unlabeled glutamic acid. It appears that the vitamin K-dependent step in the “completion” of prothrombin is carboxylation of a glutamyl residue of the preformed protein molecule. |
| Databáze: | OpenAIRE |
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