Albumin-Binding Domain Conjugate for Near-Infrared Fluorescence Lymphatic Imaging
| Autor: | Sunkuk Kwon, Cynthia Davies-Venn, Melissa B. Aldrich, Grace Wu, Barrett R. Harvey, Eva M. Sevick-Muraca, Pradip Ghosh, Bonnie Angermiller, Nathaniel Wilganowski |
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| Jazyk: | angličtina |
| Předmět: |
Indocyanine Green
Cancer Research Fluorescence-lifetime imaging microscopy Fluorophore Peptide Plasma protein binding Near-infrared fluorescence 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Lymphatic imaging Albumins Medicine & Public Health Albumin peptide conjugate Animals Humans Radiology Nuclear Medicine and imaging 030304 developmental biology Fluorescent Dyes Lymphatic Vessels chemistry.chemical_classification Medicine(all) 0303 health sciences Binding Sites Spectroscopy Near-Infrared Imaging / Radiology Imaging agent 3. Good health Transport protein Mice Inbred C57BL Spectrometry Fluorescence Oncology chemistry Biochemistry 030220 oncology & carcinogenesis Biophysics Electrophoresis Polyacrylamide Gel Peptides Binding domain Conjugate Research Article Protein Binding |
| Zdroj: | Molecular Imaging and Biology |
| ISSN: | 1536-1632 |
| DOI: | 10.1007/s11307-011-0499-x |
| Popis: | Purpose The aim of this study was to develop and characterize a novel peptide imaging agent for noninvasive near-infrared fluorescence imaging of protein transport by the lymphatics. An imaging agent consisting of a cyclic albumin-binding domain (cABD) peptide, with sequence, Arg-Leu-Ile-Glu-Asp-Ile-Cys-Leu-Pro-Arg-Trp-Gly-Cys-Leu-Trp-Glu-Asp-Asp-Lys, was conjugated to a near-infrared fluorophore, IRDye800CW, allowing for enhanced vascular uptake, retention, and fluorescence imaging. Procedure Characterization of the cABD-IRDye800 peptide conjugate was performed using fluorescence spectroscopy to assess optical properties and SDS-PAGE and Biacore binding assays to determine binding affinity and specificity. Fluorescence imaging of normal C57BL/6 mice was conducted to monitor lymphatic uptake and retention. Results cABD-IRDye800 exhibited approximately six times greater fluorescent yield and greater stability than indocyanine green, an agent previously used in humans to image lymphatic vasculature. The agent exhibited affinity for albumin with IC50 and Kd in the nanomolar range and demonstrated superior retention characteristics within mouse lymphatics when compared with IRDye800CW. Conclusions cABD-IRDye800 has utility for assessing lymphatic function in mouse models of human lymphatic disease and the potential for use in clinical diagnostic imaging of the lymphatic vasculature. Electronic supplementary material The online version of this article (doi:10.1007/s11307-011-0499-x) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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