Gambogic acid potentiates the chemosensitivity of colorectal cancer cells to 5-fluorouracil by inhibiting proliferation and inducing apoptosis
| Autor: | Zhuanpeng Chen, Wanglin Li, Chengxing Wang, Shanqi Zeng, Jie Cao, Ping Yang, Jianchang Wei, He Hu, Feng He, Huacui Chen, Junbin Zhong, Di Huang, Tong Zhang |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Cancer Research Cell growth Cell General Medicine Articles Biology Cell cycle Thymidylate synthase digestive system diseases 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Immunology and Microbiology (miscellaneous) chemistry Apoptosis 030220 oncology & carcinogenesis Survivin medicine biology.protein Cancer research Gambogic acid Growth inhibition |
| Zdroj: | Experimental and therapeutic medicine. 13(2) |
| ISSN: | 1792-0981 |
| Popis: | Chemotherapy using 5-fluorouracil (5-FU) for colorectal cancer (CRC) has low specificity and response rates, leading to severe side effects. Gambogic acid (GA), a traditional Chinese medicine, has multi-targeted anticancer effects, including growth inhibition and apoptosis induction. However, it is unclear whether a combination of 5-FU and GA has synergistic anticancer effects in CRC cells. In this study, SW480 and HCT116 human CRC cells and human intestinal epithelial cells (IECs) were treated with different concentrations of 5-FU, GA or 5-FU+GA. A Cell Counting kit-8 assay was conducted to quantify cell proliferation. The combination index (CI) was calculated and the median-effect principle was applied to analyze the interaction between 5-FU and GA. Flow cytometry was used to determine the percentage of cells undergoing apoptosis. Reverse transcription-quantitative polymerase chain reaction and western blotting were applied to measure P53, survivin and thymidylate synthase (TS) mRNA and protein levels. It was found that 5-FU+GA more pronouncedly inhibited cell growth and induced apoptosis, compared with either monotherapy. CI values |
| Databáze: | OpenAIRE |
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