PTEN regulates motility but not directionality during leukocyte chemotaxis
| Autor: | Sonia Jiménez-Baranda, Ana C. Carrera, Carlos Martínez-A, Emilia Mira, Concepción Gómez-Moutón, Santos Mañes, Domingo F. Barber, Rosa Ana Lacalle |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Cytoplasm
DNA Complementary Time Factors Cell Blotting Western Green Fluorescent Proteins HL-60 Cells Jurkat cells Catalysis Jurkat Cells Phosphatidylinositol 3-Kinases Cytosol Membrane Microdomains Cell Movement medicine Leukocytes PTEN Tensin Humans Cloning Molecular RNA Small Interfering Actin Microscopy Video biology Chemotactic Factors Dose-Response Relationship Drug Chemotaxis Tumor Suppressor Proteins PTEN Phosphohydrolase Cell Biology Actins Phosphoric Monoester Hydrolases Cell biology Protein Structure Tertiary medicine.anatomical_structure Microscopy Fluorescence biology.protein Leukocyte chemotaxis Subcellular Fractions |
| Zdroj: | Journal of cell science. 117(Pt 25) |
| ISSN: | 0021-9533 |
| Popis: | The localization at opposite cell poles of phosphatidylinositol-3 kinases and PTEN (phosphatase and tensin homolog on chromosome 10) governs Dictyostelium chemotaxis. To study this model in mammalian cells, we analyzed the dynamic redistribution of green fluorescent protein (GFP)-tagged PTEN chimeras during chemotaxis. N- or C-terminus GFP-tagged PTEN was distributed homogenously in the cytoplasm of chemotaxing PTEN-negative Jurkat cells and PTEN-positive HL60 cells. Moreover, we did not detect uropod accumulation of endogenous PTEN in chemoattractant-stimulated HL60 cells. Cell fractionation indicated that both endogenous and ectopically expressed PTEN were confined largely to the cytosol, and that chemoattractant stimulation did not alter this location. PTEN re-expression in Jurkat cells or PTEN depletion by specific siRNA in HL60 cells did not affect cell gradient sensing; PTEN nonetheless modulated chemoattractant-induced actin polymerization and the speed of cell movement. The results suggest a role for PTEN in regulating actin polymerization, but not directionality during mammalian cell chemotaxis. |
| Databáze: | OpenAIRE |
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