A novel approach to oral iron delivery using ferrous sulphate loaded solid lipid nanoparticles
| Autor: | Derek Renshaw, Naba Elsaid, Sebastien Farnaud, Francisco Corral López, Satyanarayana Somavarapu, Timothy L Jackson, M. Gulrez Zariwala |
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| Rok vydání: | 2013 |
| Předmět: |
biology
Stereochemistry Cell Survival Iron Pharmaceutical Science Nanoparticle Administration Oral Lipids Ferrous Compounds Chitosan Ferritin chemistry.chemical_compound Drug Delivery Systems chemistry Caco-2 Solid lipid nanoparticle biology.protein Particle Humans Nanoparticles Stearic acid Caco-2 Cells Nuclear chemistry |
| Zdroj: | International journal of pharmaceutics. 456(2) |
| ISSN: | 1873-3476 |
| Popis: | Iron (Fe) loaded solid lipid nanoparticles (SLN's) were formulated using stearic acid and iron absorption was evaluated in vitro using the cell line Caco-2 with intracellular ferritin formation as a marker of iron absorption. Iron loading was optimised at 1% Fe (w/w) lipid since an inverse relation was observed between initial iron concentration and SLN iron incorporation efficiency. Chitosan (Chi) was included to prepare chitosan coated SLN's. Particle size analysis revealed a sub-micron size range (300.3 ± 31.75 nm to 495.1 ± 80.42 nm), with chitosan containing particles having the largest dimensions. As expected, chitosan (0.1%, 0.2% and 0.4% w/v) conferred a net positive charge on the particle surface in a concentration dependent manner. For iron absorption experiments equal doses of Fe (20 μM) from selected formulations (SLN-FeA and SLN-Fe-ChiB) were added to Caco-2 cells and intracellular ferritin protein concentrations determined. Caco-2 iron absorption from SLN-FeA (583.98 ± 40.83 ng/mg cell protein) and chitosan containing SLN-Fe-ChiB (642.77 ± 29.37 ng/mg cell protein) were 13.42% and 24.9% greater than that from ferrous sulphate (FeSO4) reference (514.66 ± 20.43 ng/mg cell protein) ( p ≤ 0.05). We demonstrate for the first time preparation, characterisation and superior iron absorption in vitro from SLN's, suggesting the potential of these formulations as a novel system for oral iron delivery. |
| Databáze: | OpenAIRE |
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