Influence of electrical properties on the evaluation of the surface hydrophobicity of Bacillus subtilis
| Autor: | Michel Paquot, Paul Rouxhet, Philippe Jacques, François Ahimou, Philippe Thonart |
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| Rok vydání: | 2001 |
| Předmět: |
Microbiology (medical)
biology Strain (chemistry) Chemistry Surface Properties Hydrophilic interaction chromatography Analytical chemistry Bacillus subtilis Adhesion biology.organism_classification Chromatography Agarose Microbiology Bacterial Adhesion Spore Membrane Potentials Contact angle Electrokinetic phenomena Chemical engineering Zeta potential Molecular Biology Toluene |
| Zdroj: | Journal of microbiological methods. 45(2) |
| ISSN: | 0167-7012 |
| Popis: | The surface hydrophobicity of nine Bacillus subtilis strains in different states (spores, vegetative cells, and dead cells) was assessed by water contact angle measurements, hydrophobic interaction chromatography (HIC) and bacterial adhesion to hydrocarbon (BATH). Electrokinetic properties of B, subtilis strains were characterized by zeta potential measurements and found to differ appreciably according to the strain, Correlations between HIC data, BATH data and zeta potential showed that HIC and RATH are influenced by electrostatic interactions. Water contact angle measurements thus provide a better estimate of cell surface hydrophobicity. The water contact angle of B. subtilis varied according to the strain and the state, the spores tending to be more hydrophobic than vegetative cells. (C) 2001 Elsevier Science B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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