Evidence for extensive heterotrophic metabolism, antioxidant action, and associated regulatory events during winter hardening in Sitka spruce
| Autor: | Jason A. Holliday, Curtis Klumas, Elijah Myers, Eva Collakova, Ruth Grene, Haktan Suren, Lenwood S. Heath |
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| Přispěvatelé: | Computer Science, School of Plant and Environmental Sciences |
| Rok vydání: | 2012 |
| Předmět: |
0106 biological sciences
Antioxidant Chloroplasts medicine.medical_treatment Acclimatization Sitka spruce Adaptation mechanisms Plant Science Biology Microarray Photosynthesis 01 natural sciences Plastid terminal oxidase Antioxidants 03 medical and health sciences Ascorbate Peroxidases Gene Expression Regulation Plant Botany medicine Cold acclimation Picea Ecosystem 030304 developmental biology Plant Proteins Visualization 0303 health sciences Carbon metabolism Primary metabolite Metabolism Mitochondria Chloroplast Cold Temperature Biochemistry 13. Climate action Thylakoid Seasons Cell walls 010606 plant biology & botany Research Article |
| Zdroj: | BMC Plant Biology |
| ISSN: | 1471-2229 |
| Popis: | Background Cold acclimation in woody perennials is a metabolically intensive process, but coincides with environmental conditions that are not conducive to the generation of energy through photosynthesis. While the negative effects of low temperatures on the photosynthetic apparatus during winter have been well studied, less is known about how this is reflected at the level of gene and metabolite expression, nor how the plant generates primary metabolites needed for adaptive processes during autumn. Results The MapMan tool revealed enrichment of the expression of genes related to mitochondrial function, antioxidant and associated regulatory activity, while changes in metabolite levels over the time course were consistent with the gene expression patterns observed. Genes related to thylakoid function were down-regulated as expected, with the exception of plastid targeted specific antioxidant gene products such as thylakoid-bound ascorbate peroxidase, components of the reactive oxygen species scavenging cycle, and the plastid terminal oxidase. In contrast, the conventional and alternative mitochondrial electron transport chains, the tricarboxylic acid cycle, and redox-associated proteins providing reactive oxygen species scavenging generated by electron transport chains functioning at low temperatures were all active. Conclusions A regulatory mechanism linking thylakoid-bound ascorbate peroxidase action with “chloroplast dormancy” is proposed. Most importantly, the energy and substrates required for the substantial metabolic remodeling that is a hallmark of freezing acclimation could be provided by heterotrophic metabolism. |
| Databáze: | OpenAIRE |
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