Protection from radiation-induced mitochondrial and genomic DNA damage by an extract ofHippophae rhamnoides
| Autor: | Arun K. Sinha, Pankaj Chaudhary, Farhat Afrin, Manju Lata Gupta, Rakesh Sharma, Yogendra Kumar Sharma, Sandeep Kumar Shukla, Indracanti Prem Kumar, Upendra K. Sharma, Namita Samanta |
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| Rok vydání: | 2006 |
| Předmět: |
Male
DNA protection Mitochondrial DNA Antioxidant Flavonols Epidemiology DNA damage Health Toxicology and Mutagenesis medicine.medical_treatment Thymus Gland In Vitro Techniques Biology DNA Mitochondrial Mice chemistry.chemical_compound Phenols Picrates Superoxides Hippophae medicine Animals Kaempferols Genetics (clinical) Hydroxyl Radical Plant Extracts Biphenyl Compounds Hippophae rhamnoides DNA Free Radical Scavengers biology.organism_classification Molecular biology Nuclear DNA Hydrazines Real-time polymerase chain reaction chemistry Gamma Rays Quercetin Oxidation-Reduction DNA Damage |
| Zdroj: | Environmental and Molecular Mutagenesis. 47:647-656 |
| ISSN: | 1098-2280 0893-6692 |
| DOI: | 10.1002/em.20251 |
| Popis: | Hippophae rhamnoides or seabuckthorn is used extensively in Indian and Tibetan traditional medicine for the treatment of circulatory disorders, ischemic heart disease, hepatic injury, and neoplasia. In the present study, we have evaluated the radioprotective potential of REC-1001, a fraction isolated from the berries of H. rhamnoides. Chemical analysis of the extract indicated that REC-1001 was approximately 68% by weight polyphenols, and contained kaempferol, isorhamnetin, and quercetin. The effect of REC-1001 on modulating radiation-induced DNA damage was determined in murine thymocytes by measuring nonspecific nuclear DNA damage at the whole genome level using the alkaline halo assay and by measuring sequence/gene-specific DNA damage both in nuclear DNA (beta-globin gene) and in mitochondrial DNA using a quantitative polymerase chain reaction. Treatment with 10 Gy resulted in a significant amount of DNA damage in the halo assay and reductions in the amplification of both the beta-globin gene and mitochondrial DNA. REC-1001 dose-dependently reduced the amount of damage detected in each assay, with the maximum protective effects observed at the highest REC-1001 dose evaluated (250 micro g/ml). Studies measuring the nicking of naked plasmid DNA further established the radioprotective effect of REC-1001. To elucidate possible mechanisms of action, the antioxidant properties and the free-radical scavenging activities of REC-1001 were evaluated. REC-1001 dose-dependently scavenged radiation-induced hydroxyl radicals, chemically-generated superoxide anions, stabilized DPPH radicals, and reduced Fe(3+) to Fe(2+). The results of the study indicate that the REC-1001 extract of H. rhamnoides protects mitochondrial and genomic DNA from radiation-induced damage. The polyphenols/flavonoids present in the extract might be responsible for the free radical scavenging and DNA protection afforded by REC-1001. |
| Databáze: | OpenAIRE |
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