Protein abundance alterations in matched sets of macroscopically normal colon mucosa and colorectal carcinoma
| Autor: | Jan Österreicher, Jiri Knizek, Aleš Macela, Peter R. Jungblut, František Langr, Jiri Stulik, Bures J, Jandík P, Kamila Koupilova, Karel Dedic |
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| Rok vydání: | 1999 |
| Předmět: |
Gene isoform
Two-dimensional gel electrophoresis Colorectal cancer Colon Normal colon Clinical Biochemistry S100 Proteins Biology medicine.disease Biochemistry Molecular biology S100A9 Analytical Chemistry S100A8 Downregulation and upregulation Cancer research medicine biology.protein Humans Protein Isoforms Electrophoresis Polyacrylamide Gel Cyclooxygenase Intestinal Mucosa Colorectal Neoplasms |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0173-0835 |
| Popis: | Our current results, aimed at the detection of protein abundance alterations that could be associated with the process of colon tumorigenesis, are summarized. The matched sets of macroscopically normal colon mucosa and colorectal carcinoma were examined by a one- or two-dimensional electrophoretic approach and proteins were identified using immunoblotting or mass spectrometry. The following results were observed: The levels of liver fatty acid-binding protein, actin-binding protein/smooth muscle protein 22-alpha and cyclooxygenase 2 were downregulated in colorectal carcinoma compared to normal colon mucosa. Conversely, the expression of a novel variant of heat shock protein70 and several members of the S100 protein family of calcium-binding proteins (two isoforms of S100A9, S100A8, S100A11 and S100A6) were upregulated in transformed colon mucosa. Despite the variations of the levels of expression of given protein among analyzed samples, all quantitative changes were found to be statistically significant (Mann-Whitney test assuming p < or = 0.05). We conclude that the proteomic approach is useful for the study of complex biological events underlying the process of colorectal tumorigenesis. |
| Databáze: | OpenAIRE |
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