Beta-lactam antibiotic biosynthetic genes have been conserved in clusters in prokaryotes and eukaryotes
| Autor: | P Browne, J Brown, David J. Smith, B Barton, John H. Bull, John M. Ward, Alison Jane Earl, J E Hodgson, M K Burnham, Geoffrey Turner |
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| Rok vydání: | 1990 |
| Předmět: |
DNA
Bacterial Genes Fungal Restriction Mapping Streptomyces clavuligerus Penicillium chrysogenum beta-Lactams Streptomyces Flavobacterium General Biochemistry Genetics and Molecular Biology Aspergillus nidulans Nucleic acid thermodynamics Species Specificity Sequence Homology Nucleic Acid Genomic library Cloning Molecular Peptide Synthases DNA Fungal Molecular Biology Gene Gene Library Genetics General Immunology and Microbiology biology General Neuroscience Penicillium Beta lactam antibiotic Nucleic Acid Hybridization biology.organism_classification Cosmids Anti-Bacterial Agents Genes Bacterial Multigene Family Cosmid Research Article |
| Zdroj: | The EMBO journal. 9(3) |
| ISSN: | 0261-4189 |
| Popis: | A cosmid clone containing closely linked beta-lactam antibiotic biosynthetic genes was isolated from a gene library of Flavobacterium sp. SC 12,154. The location within the cluster of the DNA thought to contain the gene for delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase (ACVS), the first step in the beta-lactam antibiotic biosynthetic pathway, was identified by a novel method. This DNA facilitated the isolation, by cross-hybridization, of the corresponding DNA from Streptomyces clavuligerus ATCC 27064, Penicillium chrysogenum Oli13 and Aspergillus nidulans R153. Evidence was obtained which confirmed that the cross-hybridizing sequences contained the ACVS gene. In each case the ACVS gene was found to be closely linked to other beta-lactam biosynthetic genes and constituted part of a gene cluster. |
| Databáze: | OpenAIRE |
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