Identification, evolution, and expression partitioning of miRNAs in allopolyploid Brassica napus
| Autor: | Enhui Shen, Longjiang Fan, Li Chen, Falk Hubertus Behrens, Ian Bancroft, Weidi Wang, Daguang Cai, Jie Qiu, Jinling Meng, Boulos Chalhoub, Jun Zou, Meng Ni, Chu-Yu Ye, Yang Liu, Qian-Hao Zhu, Shutao Dai, Ruiyan Li, Xiaoxue Jiang |
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| Přispěvatelé: | Zhejiang University, National Key Laboratory of Crop Genetic Improvement [China], Huazhong Agricultural University [Wuhan] (HZAU), Christian-Albrechts University of Kiel, Commonwealth Scientific and Industrial Research Organisation [Canberra] (CSIRO), Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), University of York, National Basic Research Program of China [2011CB109306/2015CB150200], Ministry of Science and Technology of China [2013IM030700], National Science Foundation of China [31100876], FNR (Fachagentur Nachwachsende Rohstoffe), Germany [22410312], DAAD, China Scholarship Council, Huazhong Agricultural University |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
allopolyploid evolution
Small RNA Physiology [SDV]Life Sciences [q-bio] Brassica Plant Science Genome expression partitioning Evolution Molecular Species Specificity Brassica rapa microRNA [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Genomic organization Genetics biology Brassica napus Chromosome Mapping food and beverages biology.organism_classification MicroRNAs RNA Plant Brassica oleracea Genome Plant Research Paper |
| Zdroj: | Journal of Experimental Botany Journal of Experimental Botany, 2015, 66 (22), pp.7241-7253. ⟨10.1093/jxb/erv420⟩ Journal of Experimental Botany, Oxford University Press (OUP), 2015, 66 (22), pp.7241-7253. ⟨10.1093/jxb/erv420⟩ |
| ISSN: | 0022-0957 1460-2431 |
| Popis: | International audience; The recently published genome of Brassica napus offers for the first time the opportunity to gain insights into the genomic organization and the evolution of miRNAs in oilseed rape. In this study, 12 small RNA libraries from two B. napus cultivars (Tapidor and Ningyou7) and their four double-haploid lines were sequenced, employing the newly sequenced B. napus genome, together with genomes of its progenitors Brassica rapa and Brassica oleracea. A total of 645 miRNAs including 280 conserved and 365 novel miRNAs were identified. Comparative analysis revealed a high level of genomic conservation of MIRNAs (75.9%) between the subgenomes of B. napus and its two progenitors' genomes, and MIRNA lost/gain events (133) occurred in B. napus after its speciation. Furthermore, significant partitioning of miRNA expressions between the two subgenomes in B. napus was detected. The data of degradome sequencing, miRNA-mediated cleavage, and expression analyses support specific interactions between miRNAs and their targets in the modulation of diverse physiological processes in roots and leaves, as well as in biosynthesis of, for example, glucosinolates and lipids in oilseed rape. These data provide a first genome-wide view on the origin, evolution, and genomic organization of B. napus MIRNAs. |
| Databáze: | OpenAIRE |
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