OPDAT1, a plastid envelope protein involved in 12-oxo-phytodienoic acid export for jasmonic acid biosynthesis in Populus
| Autor: | Keming Luo, Hongjun Meng, Nannan Li, Xin Zhao, Xiaohong Li, Qin Song |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Populus trichocarpa Physiology Mutant Cyclopentanes Plant Science 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Biosynthesis Oxylipins Plastids Plastid Plastid envelope biology Jasmonic acid fungi food and beverages biology.organism_classification Cytosol Populus 030104 developmental biology chemistry Biochemistry Fatty Acids Unsaturated Octadecanoid pathway 010606 plant biology & botany |
| Zdroj: | Tree Physiology. 41:1714-1728 |
| ISSN: | 1758-4469 |
| Popis: | Twelve-oxo-phytodienoic acid (OPDA), the cyclopentenone precursor of jasmonic acid (JA), is required for the wounding response of plants. OPDA is derived from plastid-localized α-linolenic acid (α-LeA; 18:3) via the octadecanoid pathway, and is further exported from plastids to the cytosol for JA biosynthesis. However, the mechanism of OPDA transport from plastids has yet to be elucidated. In the current study, a plastid inner envelope-localized protein, designated 12-oxo-Phtyodienoic Acid Transporter 1 (OPDAT1), was identified and shown to potentially be involved in OPDA export from plastids, in Populus trichocarpa. Torr. OPDAT1 is expressed predominantly in young leaves of P. trichocarpa. Functional expression of OPDAT1 in yeast cells revealed that OPDAT1 is involved in OPDA transport. Loss-of-function of OPDAT1 in poplar resulted in increased accumulation of OPDA in the extracted plastids and a reduction in JA concentration, whereas an OPDAT1-overexpressing line showed a reverse tendency in OPDA accumulation and JA biosynthesis. OPDAT1 transcripts were rapidly induced by mechanical wounding of leaves, and an opdat1 mutant transgenic plant displayed increased susceptibility to spider mite (Tetranychus urticae) infestation. Collectively, these data suggest that OPDAT1 is an inner envelope transporter for OPDA, and this has potential implications for JA biosynthesis in poplar under environmental stresses. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|