In vitro culture increases mechanical stability of human tissue engineered cartilage constructs by prevention of microscale scaffold buckling
| Autor: | Itai Cohen, Lawrence J. Bonassar, Stephen Kennedy, Caroline Dugopolski, Jill M. Middendorf, Joseph Siemiatkoski, Sonya Shortkroff, Lena R. Bartell |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Cartilage Articular Male Scaffold Digital image correlation Materials science 0206 medical engineering Biomedical Engineering Biophysics 02 engineering and technology Bending Matrix (biology) 03 medical and health sciences Chondrocytes Tissue engineering Humans Orthopedics and Sports Medicine Composite material Microscale chemistry Cells Cultured Tissue Engineering Tissue Scaffolds business.industry Rehabilitation Structural engineering Compression (physics) 020601 biomedical engineering 030104 developmental biology Buckling Proteoglycans business Porosity |
| Zdroj: | Journal of biomechanics. 64 |
| ISSN: | 1873-2380 |
| Popis: | Many studies have measured the global compressive properties of tissue engineered (TE) cartilage grown on porous scaffolds. Such scaffolds are known to exhibit strain softening due to local buckling under loading. As matrix is deposited onto these scaffolds, the global compressive properties increase. However the relationship between the amount and distribution of matrix in the scaffold and local buckling is unknown. To address this knowledge gap, we studied how local strain and construct buckling in human TE constructs changes over culture times and GAG content. Confocal elastography techniques and digital image correlation (DIC) were used to measure and record buckling modes and local strains. Receiver operating characteristic (ROC) curves were used to quantify construct buckling. The results from the ROC analysis were placed into Kaplan-Meier survival function curves to establish the probability that any point in a construct buckled. These analysis techniques revealed the presence of buckling at early time points, but bending at later time points. An inverse correlation was observed between the probability of buckling and the total GAG content of each construct. This data suggests that increased GAG content prevents the onset of construct buckling and improves the microscale compressive tissue properties. This increase in GAG deposition leads to enhanced global compressive properties by prevention of microscale buckling. |
| Databáze: | OpenAIRE |
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