Immobilization of native type I collagen on polypropylene fabrics as a substrate for HepG2 cell culture
| Autor: | Xiongxin Lei, Gongze Peng, Jun Weng, S.J. Li, Guifeng Zhang, Jia Zhidong, Yang Li, Qing Peng, Yi Gao, Jiyao Kang |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Materials science Biocompatibility Nonwoven fabric Surface Properties Cell Culture Techniques Biomedical Engineering Biocompatible Materials 02 engineering and technology Polypropylenes Collagen Type I Biomaterials Contact angle 03 medical and health sciences chemistry.chemical_compound Animals Humans Composite material Cell Proliferation Polypropylene Textiles Liver cell technology industry and agriculture Substrate (chemistry) Hep G2 Cells 021001 nanoscience & nanotechnology Liver Artificial Immobilized Proteins 030104 developmental biology chemistry Chemical engineering Glutaral Hepatocytes Cattle Glutaraldehyde 0210 nano-technology Type I collagen |
| Zdroj: | Journal of Biomaterials Applications. 32:93-103 |
| ISSN: | 1530-8022 0885-3282 |
| DOI: | 10.1177/0885328217709607 |
| Popis: | Background/aims The critical part of a bio-artificial liver device is establishment of a bioreactor filled with liver cells. However, it is still unclear how to maintain benign cell function while achieving the sufficient cell quantity. In the current study, we aim to establish a novel carrier for the culture of HepG2 cells, a liver cell line, by modifying polypropylene nonwoven fabrics with native type I collagen. Methods “Piranha” solution, KH-550 and glutaraldehyde subsequently were used to bridge native type I collagen and polypropylene nonwoven fabrics. The type I collagen-coupled polypropylene nonwoven fabric was characterized by XPS, SEM, ATR-FTIR and water contact angle measurement. Furthermore, the biocompatibility between HepG2 cells and fiber film is evaluated by the ability of cell proliferation, albumin secretion, as well as urea synthesis. Results The coating of collagen onto polypropylene fabrics was more efficient using the chemical covalent binding method than direct immersion, which was validated by the presence of collagen-related elements and chemical bond. The adding of collagen in polypropylene fabrics promoted hydrophilicity and HepG2 cell adherence. Additionally, enhanced cell proliferation, increased albumin secretion and urea synthesis were observed in HepG2 cells growing on collagen-coated polypropylene fabrics. Conclusions The collagen coated polypropylene nonwoven fabrics, acting as a feasible substrate for HepG2 cell culture, may be used as a promising liver cell carrier for artificial liver reactor. |
| Databáze: | OpenAIRE |
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