Expression Changes of NMDA and AMPA Receptor Subunits in the Hippocampus in rats with Diabetes Induced by Streptozotocin Coupled with Memory Impairment
| Autor: | Jiao Lv, Qi Wan, Zhong-Yi Qian, Yong-Jie Huang, Lei Zhou, Zhi-Hao Mu, Pin Ye, Ying-Ying Zou, Wang Xie, Xiao-Peng Wang, Fang Wang, Xin-Jie Liu, Zhi-Hong Yang |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Morris water navigation task Hippocampus Gene Expression AMPA receptor Neurotransmission Biochemistry Receptors N-Methyl-D-Aspartate Diabetes Mellitus Experimental Rats Sprague-Dawley 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Internal medicine Medicine Animals Receptors AMPA Receptor Maze Learning Memory Disorders business.industry musculoskeletal neural and ocular physiology Glutamate receptor General Medicine Streptozotocin Rats Protein Subunits 030104 developmental biology Endocrinology nervous system NMDA receptor business 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Neurochemical research. 44(4) |
| ISSN: | 1573-6903 |
| Popis: | Cognitive impairment in diabetes (CID) is a severe chronic complication of diabetes mellitus (DM). It has been hypothesized that diabetes can lead to cognitive dysfunction due to expression changes of excitatory neurotransmission mediated by N-methyl-D-aspartate receptors (NMDAR) and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR); however, the pathogenesis involved in this has not been fully understood, especially at early phase of DM. Here, we sought to determine the cognitive changes and aim to correlate this with the expression changes of NMDAR and AMPAR of glutamate signaling pathways in the rat hippocampus from early phase of DM and in the course of the disease progression. By Western blot analysis and immunofluorescence labeling, the hippocampus in diabetic rats showed a significant increase in protein expression NMDAR subunits NR1, NR2A and NR2B and AMPAR subunit GluR1. Along with this, behavioral test by Morris water maze showed a significant decline in their performance when compared with the control rats. It is suggested that NR1, NR2A, NR2B and GluR1are involved in learning and memory and that their expression alterations maybe correlated with the occurrence and development of CID in diabetic rats induced by streptozotocin. |
| Databáze: | OpenAIRE |
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