Antiviral mode of action of a synthetic brassinosteroid against Junin virus replication
| Autor: | Natalia Aguirre Sgalippa, Celia E. Coto, Viviana Castilla, Mariano Larzábal, Mónica B. Wachsman |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
DNA
Complementary Biology Virus Replication Antiviral Agents Giant Cells Virus Cell Line Cell Fusion Viral Proteins chemistry.chemical_compound Cricetinae Virology Chlorocebus aethiops medicine Animals Immunoprecipitation Brassinosteroid Mode of action Vero Cells Pharmacology Junin virus Arenavirus Reverse Transcriptase Polymerase Chain Reaction RNA biology.organism_classification Cholestanones Mechanism of action chemistry Vero cell RNA Viral medicine.symptom |
| Zdroj: | Antiviral Research. 68:88-95 |
| ISSN: | 0166-3542 |
| DOI: | 10.1016/j.antiviral.2005.07.007 |
| Popis: | The antiviral mode of action of the synthetic brassinosteroid (22S,23S)-3beta-bromo-5alpha,22,23-trihydroxystigmastan-6-one (6b) against Junin virus replication in Vero cells was investigated. Time-related experiments showed that 6b mainly affects an early event of virus growth cycle. Neither adsorption nor internalization of viral particles was the target of the inhibitory action. The analysis of the effect of 6b on viral RNA synthesis demonstrated that the presence of the compound adversely affects virus RNA replication by preventing the synthesis of full length antigenomic RNA. Although 6b was most effective the earlier it was added to the cells after infection with JV, a high level of inhibition of JV yield and fusion activity of newly synthesized viral glycoproteins was still detected when the compound was present during the last hours of infection. Therefore, we cannot rule out an inhibitory action of 6b on later events of JV replicative cycle. |
| Databáze: | OpenAIRE |
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