Defects at the Posttranscriptional Level Account for the Low TCRζ Chain Expression Detected in Gastric Cancer Independently of Caspase-3 Activity
Autor: | Remedios Gómez, Ana Aguinaga-Barrilero, José Manuel Martín-Villa, Ignacio Juarez, Patricia Castro-Sánchez, Alberto Gutierrez-Calvo, Adela López, Noelia Rodríguez-Pérez |
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Rok vydání: | 2020 |
Předmět: |
Adult
Male 0301 basic medicine Article Subject T cell Immunology Receptors Antigen T-Cell Lymphocyte Activation Polymorphism Single Nucleotide Immunophenotyping Caspase 3 activity Andrology 03 medical and health sciences 0302 clinical medicine Western blot Stomach Neoplasms T-Lymphocyte Subsets Humans Immunology and Allergy Medicine RNA Processing Post-Transcriptional Aged Aged 80 and over medicine.diagnostic_test Caspase 3 business.industry Mean fluorescence intensity Cancer General Medicine RC581-607 Middle Aged medicine.disease Control subjects Gene Expression Regulation Neoplastic 030104 developmental biology medicine.anatomical_structure Mrna level Female Immunologic diseases. Allergy business Cdna sequencing Biomarkers Research Article 030215 immunology |
Zdroj: | Journal of Immunology Research Journal of Immunology Research, Vol 2020 (2020) |
ISSN: | 2314-7156 2314-8861 |
DOI: | 10.1155/2020/1039458 |
Popis: | Background. Reduced TCRζ chain surface has been reported in T cells from patients with different inflammatory conditions and cancer. However, the causes of this diminished expression in cancer remain elusive. Methods. T cell-enriched populations of blood or tissue (tumoral and nontumoral) origin from 44 patients with gastric adenocarcinoma and 33 healthy subjects were obtained. Samples were subjected to cytofluorimetry, Western blot analysis, TCRζ cDNA sequencing experiments, measurement of TCRζ mRNA levels, and caspase-3 activity assays. Results. Cytofluorimetry revealed a decreased TCRζ expression in T cells of patients, assessed either as percentage of cells expressing this chain (blood: control subjects 99.8 ± 0.1 % , patients 98.8 ± 1.1 % P < 0.001 ; tissue: control subjects 96.7 ± 0.9 % , patients tumoral tissue 67.9 ± 27.0 % , patients nontumoral tissue 82.8 ± 12.6 % , P = 0.019 ) or mean fluorescence intensity (MFI) value (blood: control subjects 102.2 ± 26.0 ; patients 58.0 ± 12.3 , P = 0.001 ; tissue: control subjects 99.4 ± 21.4 ; patients tumoral tissue 41.6 ± 21.4 ; patients nontumoral tissue 62.3 ± 16.6 , P = 0.001 ). Other chains pertaining to the TCR-CD3 complex (CD3ε) showed no significant differences (MFI values). Subsequent TCRζ cDNA sequencing experiments or measurements of TCRζ mRNA levels disclosed no differences between patients and control subjects. Evaluation of caspase-3 activity showed higher levels in T cell extracts of patients, and this activity could be decreased by 70% with the use of the inhibitor Ac-DEVD-FMK, although CD3ζ expression levels did not recover. Conclusions. These results further place the defect responsible for the low TCRζ expression in cancer at the posttranscriptional level and suggests contrary to what has been proposed in other pathologies that elevated caspase-3 activity is not the causative agent. |
Databáze: | OpenAIRE |
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