Age-related changes in the proliferative kinetics of phytohemagglutinin-stimulated lymphocytes. Analysis by uptake of tritiated precursors of DNA, RNA and proteins, and by flow cytometry
| Autor: | Lorenzo Bonomo, Adriana Dell’Osso, Giacomo Lucivero, Giuliano Mazzini, Giammarco Surico |
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| Přispěvatelé: | Lucivero, Giacomo, Surico, G, Mazzini, G, Dell'Osso, A, Bonomo, L. |
| Rok vydání: | 1988 |
| Předmět: |
Adult
Aging Lymphocyte Biology Lymphocyte Activation Tritium Umbilical cord Flow cytometry Andrology chemistry.chemical_compound Leucine Pregnancy Reference Values medicine Humans Lymphocytes Phytohemagglutinins Uridine Aged DNA synthesis medicine.diagnostic_test Infant Newborn Biological Transport DNA Fetal Blood Flow Cytometry In vitro Kinetics medicine.anatomical_structure chemistry Cell culture Protein Biosynthesis Cord blood Immunology RNA Female Thymidine Developmental Biology |
| Zdroj: | Mechanisms of Ageing and Development. 43:259-267 |
| ISSN: | 0047-6374 |
| DOI: | 10.1016/0047-6374(88)90035-8 |
| Popis: | We have examined the age-related changes in the kinetics of lymphocyte proliferative responses to phytohemagglutinin by flow cytometry and by the uptake of tritiated thymidine, uridine and leucine. The lymphocyte suspensions used in these experiments were obtained by umbilical cord blood samples of full-term normal newborns and from the peripheral blood of young (under 30-year-old) or aged (more than 70-year-old) healthy donors. The results indicate that the cord blood lymphocytes were activated by phytohemagglutinin to incorporate tritiated precursors of proteins, RNA and DNA at a more rapid rate than the lymphocytes from young or old donors in the first 3 days of in vitro culture. Flow cytometry confirmed higher percentages of activated cycling cells in umbilical cord blood after 24 h of culture. The lymphocytes from old donors incorporated significantly lower amounts of tritiated precursors of DNA and RNA than lymphocytes from young donors at the third day of culture. The uptake of tritiated leucine by lymphocytes from old donors was significantly reduced up to the sixth day of culture. On the contrary, at the eight day of culture, lymphocytes from old donors incorporated significantly higher amounts of labeled DNA, RNA and protein precursors. In agreement with these findings, flow cytometry demonstrated a trend towards higher percentages of cycling cells in the lymphocyte cultures from old donors after 6 and 8 days. These results indicate an age-related decline in the rate of lymphocyte blastogenesis after in vitro PHA stimulation and suggest that after a delayed PHA-induced activation, the lymphocytes from aged donors have a near-normal ability to proliferate. |
| Databáze: | OpenAIRE |
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