Exon junction complex components Y14 and Mago still play a role in budding yeast

Autor: Mathieu Piot, Anita Boisramé, Hugo Devillers, Juliette Pouch, Cécile Neuvéglise, François Brunel, Djamila Onésime
Přispěvatelé: MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut de biologie de l'ENS Paris (UMR 8197/1024) (IBENS), Département de Biologie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Institut de biologie de l'ENS Paris (IBENS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Département de Biologie - ENS Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), ANR-10-INBS-0009,France-Génomique,Organisation et montée en puissance d'une Infrastructure Nationale de Génomique(2010), ANR-10-BLAN-1620,YeastIntrons,Analyse globale de la régulation des ARN contenant des PTC par la voie du NMD et le complexe EJC-like chez des levures riches ou pauvres en introns(2010), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Neuvéglise, Cécile, ProdInra, Migration, Organisation et montée en puissance d'une Infrastructure Nationale de Génomique - - France-Génomique2010 - ANR-10-INBS-0009 - INBS - VALID, BLANC - Analyse globale de la régulation des ARN contenant des PTC par la voie du NMD et le complexe EJC-like chez des levures riches ou pauvres en introns - - YeastIntrons2010 - ANR-10-BLAN-1620 - BLANC - VALID, École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL), GenomiqueENS (Genomique ENS), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Département de Biologie - ENS Paris
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Saccharomyces cerevisiae Proteins
intron
RNA Splicing
[SDV]Life Sciences [q-bio]
Mutant
lcsh:Medicine
translation
[SDV.BID]Life Sciences [q-bio]/Biodiversity
Biology
nonsens-mediated decay
yarrowia-lipolytica
Article
ejc
03 medical and health sciences
0302 clinical medicine
nmd
binding-protein
cystal-structure
multiple sequence alignment
messenger-rna decay
RNA
Messenger
Saccharomycotina
lcsh:Science
ComputingMilieux_MISCELLANEOUS
Cell Nucleus
Multidisciplinary
lcsh:R
Intron
EIF4A3
RNA-Binding Proteins
Yarrowia
Translation (biology)
Exons
biology.organism_classification
Biological Evolution
Cell biology
[SDV] Life Sciences [q-bio]
030104 developmental biology
RNA splicing
Saccharomycetales
Exon junction complex
lcsh:Q
Dimerization
030217 neurology & neurosurgery
Protein Binding
Zdroj: Scientific Reports
Scientific Reports, Nature Publishing Group, 2019, 9, pp.article number 849. ⟨10.1038/s41598-018-36785-3⟩
Scientific Reports, Nature Publishing Group, 2019, 9, pp.1-18. ⟨10.1038/s41598-018-36785-3⟩
Scientific Reports, Nature Publishing Group, 2019, 9 (1), ⟨10.1038/s41598-018-36785-3⟩
Scientific Reports (9), 1-18. (2019)
Scientific Reports, Vol 9, Iss 1, Pp 1-18 (2019)
Scientific Reports, 2019, 9, pp.1-18. ⟨10.1038/s41598-018-36785-3⟩
ISSN: 2045-2322
Popis: We thank Jean-Luc Souciet, Bernard Dujon and Patrick Wincker for giving access to K. ohmeri genome sequence before publication. We thank Alex Edelman and Associates for their help in correcting the English version of the manuscript. This work was funded by the ANR YeastIntrons (ANR-2010-BLAN-1620). The École Normale Supérieure genomic core facility was supported by the “France Génomique” national infrastructure, funded as part of the “Investissements d’Avenir” program managed by the Agence Nationale de la Recherche (contract ANR-10-INBS-09). We thank Jean-Luc Souciet, Bernard Dujon and Patrick Wincker for giving access to K. ohmeri genome sequence before publication. We thank Alex Edelman and Associates for their help in correcting the English version of the manuscript. This work was funded by the ANR YeastIntrons (ANR-2010-BLAN-1620). The École Normale Supérieure genomic core facility was supported by the “France Génomique” national infrastructure, funded as part of the “Investissements d’Avenir” program managed by the Agence Nationale de la Recherche (contract ANR-10-INBS-09).; International audience; Since their divergence from Pezizomycotina, the mRNA metabolism of budding yeasts have undergone regressive evolution. With the dramatic loss of introns, a number of quality control mechanisms have been simplified or lost during evolution, such as the exon junction complex (EJC). We report the identification of the core EJC components, Mago, Y14, and eIF4A3, in at least seven Saccharomycotina species, including Yarrowia lipolytica. Peripheral factors that join EJC, either to mediate its assembly (Ibp160 or Cwc22), or trigger downstream processes, are present in the same species, forming an evolutionary package. Co-immunoprecipitation studies in Y. lipolytica showed that Mago and Y14 have retained the capacity to form heterodimers, which successively bind to the peripheral factors Upf3, Aly/REF, and Pym. Phenotypes and RNA-Seq analysis of EJC mutants showed evidence of Y14 and Mago involvement in mRNA metabolism. Differences in unspliced mRNA levels suggest that Y14 binding either interferes with pre-mRNA splicing or retains mRNA in the nucleus before their export and translation. These findings indicate that yeast could be a relevant model for understanding EJC function.
Databáze: OpenAIRE
Externí odkaz: