Propolis Extract Regulates microRNA Expression in Glioblastoma and Brain Cancer Stem Cells
| Autor: | Burak Durmaz, Ugur Yilmaz, Bakiye Goker Bagca, Sunde Yilmaz Susluer, Hüsniye Kayalar, Erhan Pariltay, Cumhur Gündüz, Emin Karaca, Asude Durmaz, Ozgur Cogulu, Ayca Aykut, Cigir Biray Avci |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Geologic Sediments
Cancer Research Turkey Cell Survival Proliferation Down-Regulation Glioma-Cells Apoptosis Biology Structure-Activity Relationship Cancer stem cell Glioma Gene expression microRNA Tumor-Suppressor Tumor Cells Cultured medicine Mechanisms Humans Bee Propolis Cell Proliferation Pharmacology Dose-Response Relationship Drug Brain Neoplasms Plant Extracts Suppresses Invasion in vitro cell culture Wnt signaling pathway glioblastoma Multiforme Cells Oncomir Propolis medicine.disease Antineoplastic Agents Phytogenic propolis MicroRNAs Chemical-Characterization Cancer research Molecular Medicine miRNA-mRNA-pathway interactions Drug Screening Assays Antitumor Stem cell Mir-146a |
| Popis: | Background: Grade IV gliomas are classified as glioblastoma (GBM), which is the most malignant brain cancer type. Various genetic and epigenetic mechanisms play a role in the initiation and progression of GBM. MicroRNAs (miRNAs) are small, non-coding RNA molecules that belong to the main epigenetic regulatory RNA class that plays different roles in either physiological or pathological conditions, including GBM pathogenesis regulating expression levels of the target genes. Brain Cancer Stem Cells (BCSCs) are responsible for poor prognosis, including therapy resistance and relapse. Epigenetic regulation mediated by miRNAs is also a critical component of BCSC self renewal and differentiation properties. Propolis is a resinous substance collected by honey bees from various plant sources. The flavonoid content of propolis varies depending on the collection region and the extraction method. Although there are studies that include the effects of different originated-propolis on the miRNA expression levels of the glioblastoma cells, the impact on the BCSCs has not been studied yet. Objective: This study aims to evaluate the effects of propolis obtained from Aydin, a city in western Turkey, on miRNA expression levels of BCSCs and GBM cells. Methods: Aydin propolis was dissolved in 60% ethanol, and after evaporation, distilled water was added to prepare the propolis stock solution. The flavonoids content of the Aydin propolis was determined by MS Q-TOF analysis. Commercially obtained U87MG and BCSCs were used as in-vitro brain cancer models. Cytotoxic and apoptotic effects of Aydin propolis were determined via WST-1 assay and Annexin V test, respectively. The miRNA expression profile was investigated using the real-time qRT-PCR method. The fold changes were calculated by the2-Delta Delta Ct method. The miRNA-mRNA-pathway interactions, including significantly altered miRNAs, were determined using different bioinformatics tools and databases. Results: Quercetin 3-methyl ether was the main component of the Aydin propolis. Aydin propolis did not show significant cytotoxic and apoptotic effects on both GBM and BCSCs up to 2mg/ml concentration. Aydin propolis treatment decreased the expression of nine miRNAs in the U87MG and five miRNAs in the BCSCs. Moreover, ten miRNAs have upregulated from 2.22 to 10.56 folds in propolis treated GBM cells compared to the control group significantly (p Ege University BAP [13-TIP-101] Y This study was partially supported by Ege University BAP with funding number 13-TIP-101. |
| Databáze: | OpenAIRE |
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