Thiol–ene micropillar array electrospray ionization platform for zeptomole level bioanalysis
| Autor: | Sari Tähkä, Sami Franssila, Ville Jokinen, Tiina Sikanen, Tapio Kotiaho, Anand Kumar Tatikonda, Risto Kostiainen, Katriina Lipponen |
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| Rok vydání: | 2017 |
| Předmět: |
Detection limit
chemistry.chemical_classification Bioanalysis Chromatography Electrospray ionization 010401 analytical chemistry technology industry and agriculture Peptide 02 engineering and technology Polyethylene glycol 021001 nanoscience & nanotechnology Mass spectrometry 01 natural sciences Biochemistry 0104 chemical sciences Analytical Chemistry Solvent chemistry.chemical_compound chemistry PEG ratio Electrochemistry Environmental Chemistry 0210 nano-technology ta116 Spectroscopy |
| Zdroj: | The Analyst. 142:2552-2557 |
| ISSN: | 1364-5528 0003-2654 |
| DOI: | 10.1039/c7an00544j |
| Popis: | A micropillar array electrospray ionization (μPESI) platform fabricated from thiol–enes with 56 individual polyethylene glycol coated μPESI chips for bioanalytical mass spectrometry is introduced. Bioanalysis capability is shown by measurement of a protein, a protein digest and a cell lysate sample. The thiol–ene polyethylene glycol (PEG) coated μPESI chip allows the use of a wide range of aqueous–organic solvent compositions and provides a detection limit at 60 zeptomole level (6 × 10−20 mol) for a peptide standard. |
| Databáze: | OpenAIRE |
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