Sulfhydryl blocker-induced colitis in the rat: Immunological changes in thymus gland and colonic mucosa
| Autor: | Mamoru Watanabe, Soichirou Miura, Mikiji Mori, Masaya Oda, Hidekazu Suzuki, Yasuo Hosoda, Shin Tanaka, Masaharu Tsuchiya, Toshifumi Hibi |
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| Rok vydání: | 1994 |
| Předmět: |
Male
Pathology medicine.medical_specialty Time Factors Colon T-Lymphocytes Fluorescent Antibody Technique Thymus Gland Immunofluorescence Immunoenzyme Techniques Rats Sprague-Dawley Atrophy Submucosa medicine Animals heterocyclic compounds Intestinal Mucosa Colitis Lamina propria Hepatology medicine.diagnostic_test business.industry Gastroenterology Flow Cytometry medicine.disease Ulcerative colitis Rats medicine.anatomical_structure Ethylmaleimide Immunohistochemistry business Infiltration (medical) |
| Zdroj: | Journal of Gastroenterology and Hepatology. 9:544-550 |
| ISSN: | 1440-1746 0815-9319 |
| DOI: | 10.1111/j.1440-1746.1994.tb01558.x |
| Popis: | The study was designed to examine the changes of thymus in sulfhydryl blocker-induced colitis. We used N-ethylmaleimide (NEM) as sulfhydryl blockers. Fasted male Sprague-Dawley rats were given 3% NEM in 1% methyl cellulose into the colon. N-ethylmaleimide treatment caused severe diarrhoea with bleeding for the first 7 days. At autopsy, adhesions, colon dilatation, and single or multiple erosions and ulcers were observed. Time-course studies revealed that the lesions were most extensive and severe 3 or 7 days after the administration of NEM. Histological examination of colon on the 3rd day after NEM treatment demonstrated mucosal erosion, oedema and extensive infiltration of neutrophils. The mucosal lesions extended into the submucosa and muscle on the 7th day after NEM treatment. Immunohistochemical studies showed that T cells and macrophages were markedly increased in the lamina propria of colonic mucosa. After 3 weeks, the infiltration of chronic inflammatory cells was observed and regeneration of the mucosa was noticed. The thymus gland was significantly decreased in weight and size on the 3rd day after NEM treatment, but the weight loss of thymus gland was regained in 3 weeks. Transient atrophy of thymus gland was noticed in this colitis model. The phenotypes of thymocytes were not influenced by NEM treatment. It is concluded that the thymus abnormalities in human ulcerative colitis are not induced in this animal model and that other chronic models are necessary for the elucidation of the immunological abnormalities, including thymus abnormalities. |
| Databáze: | OpenAIRE |
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