A novel serotonin-containing tuft cell subpopulation in mouse intestine
| Autor: | Ulrikke Voss, Xiaowen Cheng, Eva Ekblad |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Serotonin Histology Protein Serine-Threonine Kinases Tryptophan Hydroxylase Pathology and Forensic Medicine Reuptake 03 medical and health sciences Doublecortin-Like Kinases 0302 clinical medicine Intestine Small Enterochromaffin Cells medicine Animals Tuft Large intestine Intestinal Mucosa Serotonin Plasma Membrane Transport Proteins Chemistry Cell Biology Tryptophan hydroxylase Molecular biology Small intestine Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Enterochromaffin cell Tuft cell 030217 neurology & neurosurgery |
| Zdroj: | Cell and Tissue Research. 376:189-197 |
| ISSN: | 1432-0878 0302-766X |
| Popis: | In this study, a novel subset of doublecortin-like kinase 1 (DCLK1)-immunoreactive (IR) tuft cells that also contain serotonin (5-hydroxytryptamine, 5HT) is described, in terms of their number, regional distribution, possible synthesis or reuptake of 5HT and proximity to 5-HT-containing enterochromaffin (EC) cells. The small intestine from C57BL/6J mice was divided into five segments while the large intestine was kept undivided. Double immunostaining was used to estimate numbers and topographic distribution of 5HT-IR (DCLK1/5HT) tuft cells and their possible expression of tryptophan hydroxylase (TPH) and serotonin transporter (SERT). Also, possible contacts between tuft cells and 5HT-IR EC cells were studied. In the small intestine, up to 80% of all tuft cells were identified as DCLK1/5HT-IR; in the large intestine, such cells were rare. The highest number of DCLK1/5HT-IR cells was found in the upper small intestine. The numbers of DCLK1/5HT-IR cells gradually decreased distally. DCLK1-IR tuft cells were not found to contain TPH, the rate-limiting enzyme in 5HT synthesis. SERT, the selective transporter for 5HT reuptake, could not convincingly be demonstrated in tuft cells. In villi and crypts, 3% and 10%, respectively, of all DCLK1-IR cells were in close proximity to EC cells. EC cells in close proximity to DCLK1-IR cells were, in villi and crypts, 3 and 8%, respectively. We conclude that DCLK1/5HT-IR cells constitute a novel subset of tuft cells that may have unique roles in the GI tract. |
| Databáze: | OpenAIRE |
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