Effect of methylene blue photodynamic therapy on human neutrophil functional responses
| Autor: | Francesca Vita, Micaela Grandolfo, Renzo Menegazzi, Stefano Prato, Violetta Borelli, Valentina Rapozzi, Elisa Trevisan, Barbara Troian, Giuliano Zabucchi |
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| Přispěvatelé: | Trevisan, Elisa, Menegazzi, Renzo, Zabucchi, Giuliano, Troian, Barbara, Prato, Stefano, Vita, Francesca, Rapozzi, Valentina, Grandolfo, Micaela, Borelli, Violetta |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
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Human neutrophil Polymorphonuclear neutrophils Cell Survival Neutrophils medicine.medical_treatment 030303 biophysics Biophysics Photodynamic therapy 02 engineering and technology Pharmacology Cell Line 03 medical and health sciences chemistry.chemical_compound Polymorphonuclear Neutrophils Candida albicans Cell Adhesion medicine Humans Radiology Nuclear Medicine and imaging Fluorescent Dyes Peroxidase Optical LED array Antimicrobial photodynamic therapy Methylene blue Infection 0303 health sciences Oxidase test Photosensitizing Agents Radiation Radiological and Ultrasound Technology biology Optical Imaging 021001 nanoscience & nanotechnology Antimicrobial biology.organism_classification Anti-Bacterial Agents Polymorphonuclear neutrophil Photochemotherapy chemistry Myeloperoxidase biology.protein Reactive Oxygen Species 0210 nano-technology |
| Popis: | Photodynamic therapy (PDT) has become an emerging novel therapeutic approach for treating localized microbial infections, particularly those sustained by multidrug-resistant strains. Given the irreplaceable role played by professional phagocytes in limiting infections, such as polymorphonuclear neutrophils, any newly designed antimicrobial therapeutic approach must not interfere with their function. The present investigation presents a detailed analysis of the effect of PDT on the viability and several functional responses of human polymorphonuclear neutrophils loaded with methylene blue (MB), one of the more commonly used photosensitizers in antimicrobial PDT. Taking advantage of the use of a specifically-designed optical LED array for illuminating MBloaded human polymorphonuclear neutrophils, a number of cell functions have been assayed under miniaturized, strictly controlled and reproducible experimental conditions. The major findings of this study are the following: (1) MB-PDT increases human neutrophils adhesion and does not modify myeloperoxidase release; (2) MB-PDT markedly enhances reactive oxygen species generation that is independent of superoxide-forming phagocytic oxidase and very likely ascribable to LED-dependent excitation of accumulated methylene blue; (3) MB-PDT almost abolishes human neutrophils candidacidal activity by hindering the engulfing machinery. This in vitro study may represent a valuable reference point for future research on PDT applications for treating localized microbial infections. 1. Introduction Two main therapeutic approaches are nowadays available to counteract infectious diseases, i.e. (i) eliminate the microbes causing the infection and, (ii) potentiate the immune response of the affected patient. In some instances, due to the pressing need for quickly containing the ongoing infection, both therapeutic interventions are adopted at |
| Databáze: | OpenAIRE |
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