Oncotically Driven Control over Glycocalyx Dimension for Cell Surface Engineering and Protein Binding in the Longitudinal Direction
| Autor: | Donald E. Brooks, Jayachandran N. Kizhakkedathu, Erika M. J. Siren, Rafi Chapanian, Iren Constantinescu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cell type Surface Properties lcsh:Medicine CD47 Antigen Plasma protein binding Surface engineering Glycocalyx Protein Engineering Article 03 medical and health sciences Human Umbilical Vein Endothelial Cells medicine Humans lcsh:Science Multidisciplinary Chemistry Cell Membrane lcsh:R Protein engineering Red blood cell 030104 developmental biology medicine.anatomical_structure Biophysics lcsh:Q Macromolecular crowding Protein Binding Macromolecule |
| Zdroj: | Scientific Reports, Vol 8, Iss 1, Pp 1-11 (2018) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-018-25870-2 |
| Popis: | Here we present a simple technique for re-directing reactions on the cell surface to the outermost region of the glycocalyx. Macromolecular crowding with inert polymers was utilized to reversibly alter the accessibility of glycocalyx proteoglycans toward cell-surface reactive probes allowing for reactivity control in the longitudinal direction (‘z’-direction) on the glycocalyx. Studies in HUVECs demonstrated an oncotically driven collapse of the glycocalyx brush structure in the presence of crowders as the mechanism responsible for re-directing reactivity. This phenomenon is consistent across a variety of macromolecular agents including polymers, protein markers and antibodies which all displayed enhanced binding to the outermost surface of multiple cell types. We then demonstrated the biological significance of the technique by increasing the camouflage of red blood cell surface antigens via a crowding-enhanced attachment of voluminous polymers to the exterior of the glycocalyx. The accessibility to Rhesus D (RhD) and CD47 proteins on the cell surface was significantly decreased in crowding-assisted polymer grafting in comparison to non-crowded conditions. This strategy is expected to generate new tools for controlled glycocalyx engineering, probing the glycocalyx structure and function, and improving the development of cell based therapies. |
| Databáze: | OpenAIRE |
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