Specificity and Effector Functions of Human RSV-Specific IgG from Bovine Milk
| Autor: | Gerco den Hartog, Jeanette H. W. Leusen, Shamir R. Jacobino, Linda J. Cox, R. J. Joost van Neerven, Laurien H. Ulfman, Louis Bont |
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| Rok vydání: | 2014 |
| Předmět: |
Viral Diseases
acute otitis-media respiratory syncytial virus Antigen Processing and Recognition Lymphocyte Activation Antibodies Viral medicine.disease_cause Biochemistry Immune Receptors influenza-virus Immunoglobulin G Haemophilus influenzae Cell-Mediated Immunity childhood asthma Antibody Specificity Animal Cells antibody Medicine and Health Sciences Myeloid Cells Receptor Immune Response Cross Reactivity Immune System Proteins Multidisciplinary medicine.diagnostic_test biology infants Infectious Disease Immunology Hep G2 Cells protection Infectious Diseases Milk Medicine Cellular Types Antibody Research Article Science Immune Cells Immunology Antigen-Presenting Cells Celbiologie en Immunologie Antibodies Virus Flow cytometry Microbiology Immunomodulation Immune system Species Specificity Opsonization Haemophilus Fc Receptors medicine Animals Humans Mucosal Immunity Respiratory Syncytial Virus Infection Receptors IgG inverse association Immunity Biology and Life Sciences Proteins Cell Biology biology.organism_classification Virology infection Influenza Cell Biology and Immunology Respiratory Syncytial Virus Human WIAS immune-response biology.protein Clinical Immunology Cattle |
| Zdroj: | PLoS ONE PLoS ONE, Vol 9, Iss 11, p e112047 (2014) PLoS ONE, 9(11) PLoS ONE 9 (2014) 11 |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0112047 |
| Popis: | BackgroundRespiratory syncytial virus (RSV) infection is the second most important cause of death in the first year of life, and early RSV infections are associated with the development of asthma. Breastfeeding and serum IgG have been shown to protect against RSV infection. Yet, many infants depend on bovine milk-based nutrition, which at present lacks intact immunoglobulins.ObjectiveTo investigate whether IgG purified from bovine milk (bIgG) can modulate immune responses against human RSV.MethodsELISAs were performed to analyse binding of bIgG to human respiratory pathogens. bIgG or hRSV was coated to plates to assess dose-dependent binding of bIgG to human Fcγ receptors (FcγR) or bIgG-mediated binding of myeloid cells to hRSV respectively. S. Epidermidis and RSV were used to test bIgG-mediated binding and internalisation of pathogens by myeloid cells. Finally, the ability of bIgG to neutralise infection of HEp2 cells by hRSV was evaluated.ResultsbIgG recognised human RSV, influenza haemagglutinin and Haemophilus influenza. bIgG bound to FcγRII on neutrophils, monocytes and macrophages, but not to FcγRI and FcγRIII, and could bind simultaneously to hRSV and human FcγRII on neutrophils. In addition, human neutrophils and dendritic cells internalised pathogens that were opsonised with bIgG. Finally, bIgG could prevent infection of HEp2 cells by hRSV.ConclusionsThe data presented here show that bIgG binds to hRSV and other human respiratory pathogens and induces effector functions through binding to human FcγRII on phagocytes. Thus bovine IgG may contribute to immune protection against RSV. |
| Databáze: | OpenAIRE |
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