Methotrexate reduces circulating Th17 cells and impairs plasmablast and memory B cell expansions following pneumococcal conjugate immunization in RA patients
| Autor: | Åsa Pettersson, Anders A. Bengtsson, Göran Jönsson, Per Nived, Meliha C Kapetanovic, Lillemor Skattum, Åsa Johansson, Bo Settergren |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
musculoskeletal diseases Adult Male T cell Science Pneumococcal conjugate vaccine Article Flow cytometry Arthritis Rheumatoid Pneumococcal Vaccines 03 medical and health sciences 0302 clinical medicine Medicine Humans Lymphocytes Rheumatoid arthritis Memory B cell Aged 030203 arthritis & rheumatology Aged 80 and over Vaccines B-Lymphocytes Multidisciplinary medicine.diagnostic_test business.industry Vaccination Middle Aged Pneumonia Pneumococcal medicine.disease Antibodies Bacterial 030104 developmental biology medicine.anatomical_structure Methotrexate Streptococcus pneumoniae Immunization Antirheumatic Agents Case-Control Studies Immunology Antibody Formation Female business medicine.drug Conjugate |
| Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-9 (2021) |
| ISSN: | 2045-2322 |
| Popis: | Methotrexate (MTX) impairs antibody response after pneumococcal vaccination. We aimed to investigate differences in phenotypes of circulating B and T cells after pneumococcal conjugate vaccine (PCV) in rheumatoid arthritis (RA) patients on MTX (MTX group), RA without disease-modifying drugs (0DMARD), and controls (HC). MTX group (n = 11), 0DMARD (n = 12) and HC (n = 13) were studied. Blood samples were collected: before MTX, ≥ 4 weeks on stable MTX dose (prevaccination), and 7 days postvaccination (MTX group), and pre- and 7 days postvaccination (0DMARD and HC). Phenotypes of B- and T cell subsets were determined using flow cytometry. Serotype-specific IgG were quantified using multiplex bead assay, pre- and 4–6 weeks postvaccination. Concentrations of plasmablasts and switched memory B cells increased after PCV in HC (both p = 0.03) and the 0DMARD group (p = 0.01 and p = 0.02), but not in the MTX group. Postimmunization plasmablasts were lower in MTX group, compared to the 0DMARD group and HC (p = 0.002 and p p = 0.02), and increased in HC after immunization (p = 0.01). Postimmunization plasmablasts correlated with mean antibody response ratio in all RA patients (R = 0.57, p = 0.035). Methotrexate reduced Th17 cells and blocked activation of plasmablasts and switched memory B cells following polysaccharide-protein conjugate antigen challenge in RA. |
| Databáze: | OpenAIRE |
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