Short-term aromatase inhibition induces prostatic alterations in adult wistar rat: A biochemical, histopathological and immunohistochemical study
| Autor: | Reda Djidjik, Amina Cheboub, Fatima Hadj-Bekkouche, Assia Slimani, Nadia Regouat |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Amyloid Histology Time Factors medicine.drug_class medicine.disease_cause Formestane Nitric oxide 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Aromatase Prostate Internal medicine medicine Animals Rats Wistar Cell Proliferation Diminution Aromatase inhibitor business.industry Aromatase Inhibitors Tumor Suppressor Proteins Androstenedione Cell Biology General Medicine Malondialdehyde Immunohistochemistry Rats 030104 developmental biology medicine.anatomical_structure Endocrinology chemistry Gene Expression Regulation Estrogen 030220 oncology & carcinogenesis business Oxidative stress medicine.drug |
| Zdroj: | Acta histochemica. 121(8) |
| ISSN: | 1618-0372 |
| Popis: | This study aimed to evaluate the effects of estrogen reduction on amyloid deposition, some lipid metabolism and oxidative stress markers, PSA-like production and p63 expression in the prostate of the adult rat.Aromatase inhibitor: Formestane (4-OHA), was administrated to male rats, at a dose of 0.1 mg/kg b.w./day, for 10 days. The control group (CONT) received the same volume of placebo injection (NaCl 0.9%).4-OHA treatment induced a significant accumulation of intraprostatic cholesterol (138.90 ± 17.64 vs 85.12 ± 2.87, p = 0.01); against an insignificant diminution of malondialdehyde (412.6 ± 54.35 vs 842.70 ± 336.50, p 0.05) and glutathione (2.40 ± 0.23 vs 3.65 ± 0.88, p 0.05). This was associated with a significant decrease of nitric oxide (31.76 ± 7.07 vs 179.40 ± 58.35, p = 0.024). Additionally, 4-OHA significantly increased the intraprostatic production of PSA-like (11.12 ± 2.78 vs 3.91 ± 0.43, p = 0.043). The prostatic histology revealed an amyloid deposition, in all prostatic lobes and a smooth muscle layer growth (p 0.05); especially significant in the dorsal and lateral lobes. Theses lobes manifested a basal cells proliferation, with a 3-fold increase of p63 expression (p 0.001). The ventral lobe presented epithelial atrophy (37.80 ± 16.20 vs 167.60 ± 5.16, p 0.05); with occasional and significant proliferative foci (247.00 ± 9.573 vs 167.60 ± 5.16 p 0.05).Aromatase inhibition, in the adult male rat, alters the prostatic function by reducing nitric oxide availability and inducing amyloid deposition along with limiting the differentiation of basal cells, through a lobe-specific p63-overexpression. |
| Databáze: | OpenAIRE |
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