Presence of the Resistance GenesvanC1andpbp5in Phenotypically Vancomycin and Ampicillin SusceptibleEnterococcus faecalis
| Autor: | Johann Bauer, Peter Kämpf, Gabriele Mölle, Stefan Hörmansdorfer, Petra Preikschat, Ilse Bauer-Unkauf, Meike Bischoff, Karin Schwaiger, Christina Hölzel |
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| Rok vydání: | 2012 |
| Předmět: |
Microbiology (medical)
Genotype Swine Immunology Microbial Sensitivity Tests Biology Real-Time Polymerase Chain Reaction Microbiology Enterococcus faecalis Minimum inhibitory concentration Enterococcus gallinarum Bacterial Proteins Amp resistance Vancomycin Ampicillin Gene cluster medicine Animals Humans Gram-Positive Bacterial Infections Phylogeny Swine Diseases Pharmacology Vancomycin Resistance biochemical phenomena metabolism and nutrition biology.organism_classification Anti-Bacterial Agents Repressor Proteins Phenotype Ampicillin Resistance medicine.drug Enterococcus faecium |
| Zdroj: | Microbial Drug Resistance. 18:434-439 |
| ISSN: | 1931-8448 1076-6294 |
| Popis: | Ampicillin and vancomycin are important antibiotics for the therapy of Enterococcus faecalis infections. The ampicillin resistance gene pbp5 is intrinsic in Enterococcus faecium. The vanC1 gene confers resistance to vancomycin and serves as a species marker for Enterococcus gallinarum. Both genes are chromosomally located. Resistance to ampicillin and vancomycin was determined in 484 E. faecalis of human and porcine origin by microdilution. Since E. faecalis are highly skilled to acquire resistance genes, all strains were investigated for the presence of pbp5 (and, in positive strains, for the penicillin-binding protein synthesis repressor gene psr) and vanC1 (and, in positive strains, for vanXYc and vanT) by using polymerase chain reaction (PCR). One porcine and one human isolate were phenotypically resistant to ampicillin; no strain was vancomycin resistant. Four E. faecalis (3/1 of porcine/human origin) carried pbp5 (MIC=1 mg/L), and four porcine strains were vanC1 positive (minimum inhibitory concentration [MIC]=1 mg/L). Real-time reverse transcriptase (RT)-PCR revealed that the genes were not expressed. The psr gene was absent in the four pbp5-positive strains; the vanXYc gene was absent in the four vanC1-positive strains. However, vanT of the vanC gene cluster was detected in two vanC1-positive strains. To our knowledge, this is the first report on the presence of pbp5, identical with the "E. faecium pbp5 gene," and of vanC1/vanT in E. faecalis. Even if resistance is not expressed in these strains, this study shows that E. faecalis have a strong ability to acquire resistance genes-and potentially to spread them to other bacteria. Therefore, close monitoring of this species should be continued. |
| Databáze: | OpenAIRE |
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