Structural heterogeneity of the sialic-acid-containing oligosaccharides from the lipopolysaccharide of Hafnia alvei strain 2 as detected by FABMS studies
| Autor: | Małgorzata Mieszała, Danuta Witkowska, Elzbieta Romanowska, Jacques Defaye, Jacques Ulrich, Andrzej Gamian |
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| Rok vydání: | 1999 |
| Předmět: |
Lipopolysaccharides
Magnetic Resonance Spectroscopy Lipopolysaccharide Molecular Sequence Data Oligosaccharides Biochemistry Mass Spectrometry Analytical Chemistry chemistry.chemical_compound Residue (chemistry) Enterobacteriaceae Chromatography High Pressure Liquid chemistry.chemical_classification Strain (chemistry) Organic Chemistry General Medicine Nuclear magnetic resonance spectroscopy Oligosaccharide Structural heterogeneity N-Acetylneuraminic Acid Sialic acid Hafnia alvei chemistry Carbohydrate Sequence Models Chemical |
| Zdroj: | Carbohydrate research. 314(3-4) |
| ISSN: | 0008-6215 |
| Popis: | The structure of four oligosaccharide fractions from the Hafnia alvei strain 2 lipopolysaccharide (LPS) have been assigned by FABMS. This approach corroborates data previously established by NMR spectroscopy for the major oligosaccharides in these fractions [A. Gamian, E. Romanowska, U. Dabrowski, J. Dabrowski, Biochemistry 30 (1991) 5032–5038; E. Katzenellenbogen, A. Gamian, E. Romanowska, U. Dabrowski, J. Dabrowski, Biochem. Biophys. Res. Commun. 194 (1993) 1058–1064; N. Ravenscroft, A. Gamian, E. Romanowska, Eur. J. Biochem. 227 (1995) 889–896]. In addition, the MS/MS with B/E linked scan technique allowed the detection of an additional oligosaccharide with the structure: Download full-size image lacking the branched O-6 linked glucopyranose residue at the 3-linked Gal unit, which indicates a structural heterogeneity for the major oligosaccharide fraction. |
| Databáze: | OpenAIRE |
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