Antimicrobials from a feline commensal bacterium inhibit skin infection by drug-resistant S. pseudintermedius

Autor: Alan M. O’Neill, Robert H. Mills, Kate A. Worthing, Kit Pogliano, Dominic McGrosso, Richard L. Gallo, David Gonzalez, Fengwu Li, Nikhil Nitin Kulkarni, Gayathri Kalla, Teruaki Nakatsuji, Jacqueline M. Norris, Joe Pogliano, Joyce Y. Cheng
Rok vydání: 2021
Předmět:
Staphylococcus pseudintermedius
Staphylococcus
staphylococcus felis
Anti-Inflammatory Agents
Drug Resistance
Drug resistance
Skin infection
medicine.disease_cause
Mass Spectrometry
Bacteriocins
Anti-Infective Agents
B. subtilis
2.2 Factors relating to the physical environment
Biology (General)
Aetiology
biology
General Neuroscience
Bacterial
General Medicine
Staphylococcal Infections
Antimicrobial
Anti-Bacterial Agents
Infectious Diseases
Staphylococcus aureus
Medicine
Bacteriotherapy
Pore Forming Cytotoxic Proteins
skin
QH301-705.5
Science
infectious disease
General Biochemistry
Genetics and Molecular Biology
Microbiology
Vaccine Related
staphylococcus pseudintermedius
medicine
Animals
Staphylococcus felis
human
mouse
Whole Genome Sequencing
General Immunology and Microbiology
microbiology
E. coli
biology.organism_classification
medicine.disease
infection
Transplantation
Emerging Infectious Diseases
Cats
antimicrobial
Antimicrobial Resistance
Biochemistry and Cell Biology
Zdroj: eLife, Vol 10 (2021)
ISSN: 2050-084X
DOI: 10.7554/elife.66793
Popis: Methicillin-resistant Staphylococcus pseudintermedius (MRSP) is an important emerging zoonotic pathogen that causes severe skin infections. To combat infections from drug-resistant bacteria, the transplantation of commensal antimicrobial bacteria as a therapeutic has shown clinical promise. We screened a collection of diverse staphylococcus species from domestic dogs and cats for antimicrobial activity against MRSP. A unique strain (S. felis C4) was isolated from feline skin that inhibited MRSP and multiple gram-positive pathogens. Whole genome sequencing and mass spectrometry revealed several secreted antimicrobials including a thiopeptide bacteriocin micrococcin P1 and phenol-soluble modulin beta (PSMβ) peptides that exhibited antimicrobial and anti-inflammatory activity. Fluorescence and electron microscopy revealed that S. felis antimicrobials inhibited translation and disrupted bacterial but not eukaryotic cell membranes. Competition experiments in mice showed that S. felis significantly reduced MRSP skin colonization and an antimicrobial extract from S. felis significantly reduced necrotic skin injury from MRSP infection. These findings indicate a feline commensal bacterium that could be utilized in bacteriotherapy against difficult-to-treat animal and human skin infections.
Databáze: OpenAIRE
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