Microsticks as solid-phase carriers for enzyme-linked immunosorbent assays
| Autor: | John L. Sever, Isabel C. Shekarchi, L A Ward, David L. Madden |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Microbiology (medical)
Materials science Enzyme-Linked Immunosorbent Assay Antibodies Immunoenzyme Techniques Measles virus chemistry.chemical_compound Phase (matter) Humans Antigens Polycarbonate chemistry.chemical_classification Chromatography biology Coating materials biology.organism_classification Molecular biology Enzyme chemistry Immunoenzyme techniques visual_art visual_art.visual_art_medium Toxoplasma Nitrocellulose Measles Research Article |
| Zdroj: | Journal of Clinical Microbiology. 16:1012-1018 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.16.6.1012-1018.1982 |
| Popis: | Microsticks are machine-tooled or molded pegs of plastic or stainless steel which were developed as solid-phase carriers for the enzyme-linked immunosorbent assay (ELISA). They consist of a stem, which can be coated with plastic to be used as the reactive surface, and a shaft designed for easy handling and labeling and for positioning the sticks in microtiter wells and transfer plates. Microsticks permit a wide selection of coating materials and afford the user greater control over quality and standardization of the solid-phase surface. Polycarbonate and nitrocellulose coatings were tested in ELISAs for antibodies to measles virus, toxoplasma, and human gamma globulin. The Microsticks were found to give reproducible, sensitive, and specific ELISA results and minimized problems due to lot-to-lot and batch-to-batch variations found with other plastic carriers. |
| Databáze: | OpenAIRE |
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