Extracellular ATP and β-NAD alter electrical properties and cholinergic effects in the rat heart in age-specific manner
| Autor: | V. M. Potekhina, Vlad S. Kuzmin, Denis V. Abramochkin, Alexey M. Petrov, K. B. Pustovit, Alexandra Ivanova |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Action Potentials Stimulation Inhibitory postsynaptic potential Synaptic Transmission 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Adenosine Triphosphate Postsynaptic potential Internal medicine medicine Extracellular Myocyte Animals Rats Wistar Molecular Biology Chemistry Myocardium Heart Cell Biology NAD Rats Electrophysiology 030104 developmental biology Endocrinology cardiovascular system Cholinergic Female Original Article 030217 neurology & neurosurgery Acetylcholine medicine.drug |
| Zdroj: | Purinergic signalling. 15(1) |
| ISSN: | 1573-9546 |
| Popis: | Extracellular ATP and nicotinamide adenine dinucleotide (β-NAD) demonstrate properties of neurotransmitters and neuromodulators in peripheral and central nervous system. It has been shown previously that ATP and β-NAD affect cardiac functioning in adult mammals. Nevertheless, the modulation of cardiac activity by purine compounds in the early postnatal development is still not elucidated. Also, the potential influence of ATP and β-NAD on cholinergic neurotransmission in the heart has not been investigated previously. Age-dependence of electrophysiological effects produced by extracellular ATP and β-NAD was studied in the rat myocardium using sharp microelectrode technique. ATP and β-NAD could affect ventricular and supraventricular myocardium independent from autonomic influences. Both purines induced reduction of action potentials (APs) duration in tissue preparations of atrial, ventricular myocardium, and myocardial sleeves of pulmonary veins from early postnatal rats similarly to myocardium of adult animals. Both purine compounds demonstrated weak age-dependence of the effect. We have estimated the ability of ATP and β-NAD to alter cholinergic effects in the heart. Both purines suppressed inhibitory effects produced by stimulation of intracardiac parasympathetic nerve in right atria from adult animals, but not in preparations from neonates. Also, ATP and β-NAD suppressed rest and evoked release of acetylcholine (ACh) in adult animals. β-NAD suppressed effects of parasympathetic stimulation and ACh release stronger than ATP. In conclusion, ATP and β-NAD control the heart at the postsynaptic and presynaptic levels via affecting the cardiac myocytes APs and ACh release. Postsynaptic and presynaptic effects of purines may be antagonistic and the latter demonstrates age-dependence. |
| Databáze: | OpenAIRE |
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