Steroid hormone biosynthesis by a sesterterpene pathway in the rat and rabbit testis
| Autor: | K.B. Eik-Nes, A.D. Tait, L.C. Hodge |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Male
medicine.medical_specialty Sesterterpenes Steroid biosynthesis Biology Testicle Biochemistry chemistry.chemical_compound Endocrinology Biosynthesis Microsomes Internal medicine Testis medicine Animals Testosterone Androstenedione Terpenes Cholesterol Mitochondria Rats medicine.anatomical_structure chemistry Pregnenolone Microsome Specific activity Rabbits |
| Zdroj: | Journal of Steroid Biochemistry. 26:99-103 |
| ISSN: | 0022-4731 |
| DOI: | 10.1016/0022-4731(87)90036-7 |
| Popis: | Rat and rabbit testis preparations were incubated with [4-14C]cholesterol and 23,24-dinor-[7 alpha-3H]5-cholen-3 beta-ol, the latter being a proposed intermediate in the sesterterpene pathway for steroid biosynthesis. Steroids were isolated, purified by thin-layer chromatography and crystallised to constant specific activity. It was found that rat and rabbit testis can utilise 23,24-dinor-5-cholen-3 beta-ol to produce testosterone. The tritium/carbon-14 ratios in the testosterone and androstenedione isolated indicated that these tissues differentiated between the two substrates. This finding is supported by the observation that, on stimulation with HCG, the tritium/carbon-14 ratios in the testosterone isolated were increased compared to the controls. The results of further experiments implied that, while the biosynthesis of testosterone from cholesterol occurred in the rat testis mitochondrial fraction, its biosynthesis from 23,24-dinor-5-cholen-3 beta-ol occurred in the microsomal fraction. |
| Databáze: | OpenAIRE |
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