Novel LNA probe-based assay for the A1 and A2 identification of β-casein gene in milk samples
| Autor: | Bianca Tainá Azevedo, Anibal Eugênio Vercesi Filho, Márcia Cristina de Sena Oliveira, Luciana Morita Katiki, Gunta Gutmanis, Cintia Hiromi Okino, Rodrigo Giglioti |
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| Přispěvatelé: | RODRIGO GIGLIOTI, IZ, CINTIA HIROMI OKINO, CPPSE, BIANCA TAINA AZEVEDO, IZ, GUNTA GUTMANIS, IZ, LUCIANA MORITA KATIKI, IZ, MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE, ANIBAL EUGENIO VERCESI FILHO, IZ. |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA Food Chemistry: Molecular Sciences, Vol 3, Iss, Pp 100055-(2021) |
| ISSN: | 2666-5662 |
| DOI: | 10.1016/j.fochms.2021.100055 |
| Popis: | The rising consumption of A2 milk and its derivatives in recent years has garnered attention from both consumers and producers, mainly due its possible health benefits, such as enhanced digestion and easier absorption. Thus, a novel real-time PCR using a combination of locked nucleic acid modified (LNA) conjugated probes was developed to genotype A1 and A2 alleles of B-casein gene (CSN2) and to detect and quantify the A1 presence in A2 samples. The limit of detection for each probe (A1 and A2) was evaluated using decreasing serial dilutions. Besides, the sensitivity of A1 allele detection in the A2 samples was also tested. The limits of detection of A1 and A2 alleles were 6 copies, while for A1 allele detection in A2 samples was 7.5 copies (1%). The LNA-probe based method was found to be rapid, robust, highly sensitive, cost effective, and can be employed as screening test to certificate the A2 dairy products. Made available in DSpace on 2021-12-06T17:00:26Z (GMT). No. of bitstreams: 1 NovelLNAProbe.pdf: 2350901 bytes, checksum: d94376dbe44edbb2d6aa5aacbddc0313 (MD5) Previous issue date: 2021 |
| Databáze: | OpenAIRE |
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