PE11, a PE/PPE family protein of Mycobacterium tuberculosis is involved in cell wall remodeling and virulence
| Autor: | Sandeep Kumar Kotturu, J. R. C. Reddy, Sangita Mukhopadhyay, Rameshwaram Nagender Rao, Sudip Ghosh, Parul Singh, Rachapudi B. N. Prasad |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Virulence Factors medicine.drug_class Transgene Mycobacterium smegmatis 030106 microbiology Antibiotics Gene Expression Mycobacterium Infections Nontuberculous Virulence Biology Article Microbiology Mycobacterium tuberculosis Mice 03 medical and health sciences Glycolipid Bacterial Proteins Cell Wall Gene expression medicine Animals Transgenes Lung Mice Inbred BALB C Multidisciplinary Organisms Genetically Modified Tumor Necrosis Factor-alpha Macrophages biology.organism_classification Survival Analysis Pathophysiology Interleukin-10 030104 developmental biology Liver Immunology Interleukin-4 Cell envelope |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | The role of the unique proline-glutamic acid (PE)/proline-proline-glutamic acid (PPE) family of proteins in the pathophysiology and virulence of Mycobacterium tuberculosis is not clearly understood. One of the PE family proteins, PE11 (LipX or Rv1169c), specific to pathogenic mycobacteria is found to be over-expressed during infection of macrophages and in active TB patients. In this study, we report that M. smegmatis expressing PE11 (Msmeg-PE11) exhibited altered colony morphology and cell wall lipid composition leading to a marked increase in resistance against various environmental stressors and antibiotics. The cell envelope of Msmeg-PE11 also had greater amount of glycolipids and polar lipids. Msmeg-PE11 was found to have better survival rate in infected macrophages. Mice infected with Msmeg-PE11 had higher bacterial load, showed exacerbated organ pathology and mortality. The liver and lung of Msmeg-PE11-infected mice also had higher levels of IL-10, IL-4 and TNF-α cytokines, indicating a potential role of this protein in mycobacterial virulence. |
| Databáze: | OpenAIRE |
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