The sigma ligand, igmesine, inhibits cholera toxin and Escherichia coli enterotoxin induced jejunal secretion in the rat
| Autor: | J L Turvill, P Kasapidis, M J G Farthing |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Cyclopropanes
Male Cholera Toxin Bacterial Toxins Sigma receptor Enterotoxin Pharmacology Biology Ligands medicine.disease_cause Article Microbiology Electrolytes Enterotoxins Igmesine Body Water In vivo medicine Animals Secretion Rats Wistar Toxin Escherichia coli Proteins Cholera toxin Gastroenterology medicine.disease Cholera Rats Jejunum Cinnamates Antitoxins |
| Zdroj: | Gut. 45:564-569 |
| ISSN: | 0017-5749 |
| Popis: | BACKGROUND—Cholera toxin, and Escherichia coli heat labile (LT) and heat stable (STa) enterotoxins induce small intestinal secretion in part by activating enteric nerves. Igmesine is a novel sigma receptor ligand that inhibits neurally mediated secretion. AIMS—To assess the antisecretory potential of igmesine in cholera toxin, LT, and STa induced water and electrolyte secretion using an in vivo rat model of jejunal perfusion. METHODS—After pretreatment with igmesine, 0.03-10 mg/kg intravenously, jejunal segments of anaesthetised, adult male Wistar rats were incubated with cholera toxin (25 µg), LT (25 µg), or saline. Jejunal perfusion with a plasma electrolyte solution containing a non-absorbable marker was undertaken. In some cases 200 µg/l STa was added to the perfusate. After equilibration, net water and electrolyte movement was determined. In additional experiments rats received igmesine, intravenously or intrajejunally, after exposure to cholera toxin. RESULTS—Cholera toxin induced net water secretion was inhibited by 1 mg/kg igmesine (median −120 versus −31 µl/min/g, p |
| Databáze: | OpenAIRE |
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