Equine sarcoid fibroblasts over-express matrix metalloproteinases and are invasive
| Autor: | Philipe A.M. Gobeil, ZhengQiang Yuan, M. Saveria Campo, Lubna Nasir |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Skin Neoplasms
Matrix metalloproteinase GM6001 Viral Proteins chemistry.chemical_compound stomatognathic system Cell Line Tumor hemic and lymphatic diseases Virology medicine Animals Gelatinase Neoplasm Invasiveness Horses Gene Bovine papillomavirus 1 Bovine papillomavirus biology Matrigel Invasion Assay Fibroblasts biology.organism_classification Matrix Metalloproteinases DNA-Binding Proteins stomatognathic diseases Matrix Metalloproteinase 9 chemistry Cell culture Immunology Collagenase Cancer research Cattle Horse Diseases Matrix Metalloproteinase 1 medicine.drug |
| Zdroj: | Virology. 396:143-151 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/j.virol.2009.10.010 |
| Popis: | Papillomaviruses are DNA viruses that cause tumours of the skin in humans and animals. The natural host of bovine papillomavirus is cattle, but also equids, resulting in tumours termed sarcoids. Matrix metalloproteinase 1 (MMP-1) expression is up-regulated in sarcoid fibroblasts and tumours. We extended our observation to other MMPs and determined whether MMPs induced invasion of sarcoid fibroblasts. Collagenase (MMP-1) and Gelatinase (MMP-2, MMP-9) were over-expressed in sarcoid fibroblasts and tumours. The fibroblasts were invasive in a 3D/matrigel invasion assay system. Inhibition of MMP by GM6001 significantly reduced invasion. E2 siRNA treatment of sarcoid fibroblasts decreased the expression of the viral genes and of MMP-2 and -9, leading to a dramatic reduction of invasion. This demonstrates that BPV-1 induces over-expression of MMPs contributing to invasiveness of sarcoid fibroblasts. Inhibition of E2 by siRNA leads to abrogation of invasion suggesting that E2 is a good target for sarcoid treatment. |
| Databáze: | OpenAIRE |
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