In situ detection of hepatitis A virus in cell cultures and shellfish tissues
| Autor: | T G Metcalf, Mary K. Estes, C M Woodley, Robert L. Atmar, G Szücs, Jesús L. Romalde |
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| Jazyk: | angličtina |
| Rok vydání: | 1994 |
| Předmět: |
In situ
Transcription Genetic viruses Molecular Sequence Data Biology Applied Microbiology and Biotechnology Virus Transcription (biology) Complementary DNA Animals Hepatovirus Shellfish Cells Cultured Ecology Base Sequence RNA-Directed DNA Polymerase RNA Haplorhini Virology Molecular biology Ostreidae Cell culture Food Science Biotechnology Research Article |
| Zdroj: | Scopus-Elsevier |
| Popis: | An in situ transcription method was developed to detect hepatitis A virus RNA in both cell cultures and shellfish tissues. Radiolabeled cDNA copies were synthesized in situ by reverse transcriptase-directed transcription after annealing with a specific primer to the viral RNA. Both tritium (3H) and 35S were useful in the in situ transcription reaction, but the use of 3H resulted in a lower background and finer detail in the localization of viral particles. Application of the method to different organs of oysters which had bioaccumulated hepatitis A virus allowed the first in situ localization of the virus, specifically in stomach and hepatopancreatic tissues. |
| Databáze: | OpenAIRE |
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