Retrofractamide C Derived from Piper longum Alleviates Xylene-Induced Mouse Ear Edema and Inhibits Phosphorylation of ERK and NF-κB in LPS-Induced J774A.1

Autor:	Seung Woong Lee, Soyoung Lee, Seung-Jae Lee, Sae-Kwang Ku, Hyung Jin Lim, Kang-Min Lee, Mun-Chual Rho, Seon Gyeong Bak, Eun Jae Park
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	MAPK/ERK pathway p38 mitogen-activated protein kinases Pharmaceutical Science Analytical Chemistry Nitric oxide lcsh:QD241-441 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine lcsh:Organic chemistry Piper longum Drug Discovery retrofractamide C Physical and Theoretical Chemistry 030304 developmental biology 0303 health sciences biology Kinase Organic Chemistry NF-κB Molecular biology anti-inflammation chemistry Chemistry (miscellaneous) 030220 oncology & carcinogenesis biology.protein xylene-induced ear edema Molecular Medicine Phosphorylation lipids (amino acids peptides and proteins) Cyclooxygenase Signal transduction
Zdroj:	Molecules Volume 25 Issue 18 Molecules, Vol 25, Iss 4058, p 4058 (2020)
ISSN:	1420-3049
DOI:	10.3390/molecules25184058
Popis:	Many studies have reported the biological activities of retrofractamide C (RAC). However, few studies have investigated the anti-inflammatory effect of RAC. In the present study, we investigated the anti-inflammatory effect of RAC using lipopolysaccharide (LPS)-induced J774A.1 cells and a xylene-induced mouse ear edema model. Treatment with RAC decreased LPS-induced nitric oxide (NO) and prostaglandin E2 (PGE2) secretion and inducible NO synthase (iNOS) and cyclooxygenase 2 (COX2) protein expression. It also downregulated the LPS-induced production of interleukin-1&beta (IL-1&beta ) and interleukin-6 (IL-6) but not tumor necrosis factor &alpha (TNF-&alpha ). In the LPS-induced signaling pathway, RAC inhibited the phosphorylation of extracellular signal-regulated kinase (ERK) and nuclear factor kappa light chain enhancer of activated B cells (NF-&kappa B) but not c-Jun N-terminal kinase (JNK) or p38. In a xylene-induced mouse ear edema model, RAC treatment alleviated edema formation and inflammatory cell infiltration. In conclusion, the present study indicates that RAC has the potential to have anti-inflammatory effects and could be a prospective functional food.
Databáze:	OpenAIRE
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