In vitro fidelity of the prototype primate foamy virus (PFV) RT compared to HIV-1 RT
| Autor: | Paul L. Boyer, Carolyn R. Stenbak, Stephen H. Hughes, Maxine L. Linial, David Hoberman |
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| Rok vydání: | 2007 |
| Předmět: |
Primates
Deoxyribonucleotides medicine.disease_cause Article Virus Structure-Activity Relationship 03 medical and health sciences Virology Reverse transcriptase medicine Animals Missense mutation Spumavirus Polymerase 030304 developmental biology 0303 health sciences Mutation biology Lentivirus 030302 biochemistry & molecular biology biology.organism_classification Molecular biology HIV Reverse Transcriptase In vitro 3. Good health Fidelity HIV-1 biology.protein RNA Viral |
| Zdroj: | Virology. 367:253-264 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/j.virol.2007.05.034 |
| Popis: | We compared the in vitro fidelity of wild-type human immunodeficiency virus type-1 (HIV-1) reverse transcriptase (RT) and the prototype foamy virus (PFV) RT. Both enzymes had similar error rates for single nucleotide substitutions; however, PFV RT did not appear to make errors at specific hotspots, like HIV-1 RT. In addition, PFV RT made more deletions and insertions than HIV-1 RT. Although the majority of the missense errors made by HIV-1 RT and PFV RT are different, relatively few of the mutations caused by either enzyme can be explained by a misalignment/slippage mechanism. We suggest that the higher polymerase activity of PFV RT could contribute to the ability of the enzyme to jump to the same or a different template. |
| Databáze: | OpenAIRE |
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