Diet and exercise modulate gh-igfs axis, proteolytic markers and myogenic regulatory factors in juveniles of gilthead sea bream (Sparus aurata)
| Autor: | Isabel Achaerandio, Miquel Perelló-Amorós, Arnau Innamorati, Joaquim Gutiérrez, Montserrat Pujolà, Isabel García-Pérez, Josep A. Calduch-Giner, Jaume Pérez-Sánchez, Emilio J. Vélez, Josefina Blasco, Jaume Fernández-Borràs, Albert Sánchez-Moya |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (España), Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Generalitat de Catalunya, Department of Cellular Biology, Physiology and Immunology [Barcelona, Spain], Autonomous University of Barcelona, Nutrition, Métabolisme, Aquaculture (NuMéA), Université de Pau et des Pays de l'Adour (UPPA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Universitat Politècnica de Catalunya [Barcelona] (UPC), Nutrigenomics and Fish Growth Endocrinology Group, Institute of Aquaculture Torre la Sal (IATS), 'Ministerio de Economia y Competitividad' (MINECO) AGL2015-70679-R / RTI2018-100757-B-I00, Generalitat de Catalunya General Electric 2017SGR1574, MINECO BES-2016-078697 / PRE2019-089578 / BES-2013-062949, Xarxa de Referencia d'R+D+I en Aquicultura, Universitat Politècnica de Catalunya. Departament d'Enginyeria Agroalimentària i Biotecnologia, Universitat Politècnica de Catalunya. GINEMQUAL - Gestió Integrada de Nematodes Fitoparàsits i dels Efectes sobre el Rendiment i Qualitat de la Collita |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Proteases Veterinary medicine 030209 endocrinology & metabolism Biology Growth hormone hypercaloric-diets Article 03 medical and health sciences 0302 clinical medicine Nutrient Sparus aurata Internal medicine SF600-1100 medicine fillet quality Aerobic exercise Endocrine system 14. Life underwater Muscle remodeling Gene Enginyeria agroalimentària::Indústries agroalimentàries::Indústria del peix [Àrees temàtiques de la UPC] Aerobic training Hypercaloric-diets General Veterinary [SDV.BA]Life Sciences [q-bio]/Animal biology Plasma gh endocrine regulation Fillet quality 030104 developmental biology Endocrinology QL1-991 Endocrine regulation Myogenic regulatory factors muscle remodeling Agrotech Animal Science and Zoology Dieta aerobic training Zoology [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Animals Volume 11 Issue 8 Animals, MDPI, 2021, 11 (8), ⟨10.3390/ani11082182⟩ Animals : an Open Access Journal from MDPI Animals, Vol 11, Iss 2182, p 2182 (2021) UPCommons. Portal del coneixement obert de la UPC Universitat Politècnica de Catalunya (UPC) |
| ISSN: | 2076-2615 |
| DOI: | 10.3390/ani11082182⟩ |
| Popis: | © 2021 by the authors. The physiological and endocrine benefits of sustained exercise in fish were largely demonstrated, and this work examines how the swimming activity can modify the effects of two diets (high-protein, HP: 54% proteins, 15% lipids; high-energy, HE: 50% proteins, 20% lipids) on different growth performance markers in gilthead sea bream juveniles. After 6 weeks of experimentation, fish under voluntary swimming and fed with HP showed significantly higher circulating growth hormone (GH) levels and plasma GH/insulin-like growth-1 (IGF-1) ratio than fish fed with HE, but under exercise, differences disappeared. The transcriptional profile of the GH-IGFs axis molecules and myogenic regulatory factors in liver and muscle was barely affected by diet and swimming conditions. Under voluntary swimming, fish fed with HE showed significantly increased mRNA levels of capn1, capn2, capn3, capns1a, n3, and ub, decreased gene and protein expression of Ctsl and Mafbx and lower muscle texture than fish fed with HP. When fish were exposed to sustained exercise, diet-induced differences in proteases’ expression and muscle texture almost disappeared. Overall, these results suggest that exercise might be a useful tool to minimize nutrient imbalances and that proteolytic genes could be good markers of the culture conditions and dietary treatments in fish. This study was supported by the projects from the “Ministerio de Economía y Competitividad” (MINECO) AGL2015-70679-R and RTI2018-100757-B-I00 to J.G. and J.B., and the “Xarxa de Refèrencia d’R+D+I en Aqüicultura” and the 2017SGR1574 from the “Generalitat de Catalunya”. M.P.- A., I.G.-P. and E.J.V. were supported by predoctoral fellowships from the MINECO, BES-2016-078697, PRE2019-089578 and BES-2013-062949, respectively. |
| Databáze: | OpenAIRE |
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