Gene Expression Analysis in Four Dogs With Canine Pemphigus Clinical Subtypes Reveals B Cell Signatures and Immune Activation Pathways Similar to Human Disease
| Autor: | Ramón M. Almela, Diana Junyue Ma, Haya S. Raef, Nicholas A. Robinson, Cesar Piedra-Mora, Jillian M. Richmond, Neil B. Wong, Clément N. David |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Pemphigoid
Medicine (General) skin Mucocutaneous zone Biology CD19 Immune system R5-920 immune system diseases medicine NanoString cytokine CXCL10 skin and connective tissue diseases B cell comparative immunology integumentary system Acantholysis autoimmune blistering diseases pemphigus General Medicine Brief Research Report medicine.disease canine (dog) Pemphigus medicine.anatomical_structure Immunology biology.protein gene expression Medicine |
| Zdroj: | Frontiers in Medicine Frontiers in Medicine, Vol 8 (2021) |
| ISSN: | 2296-858X |
| Popis: | Pemphigus is a group of autoimmune-mediated mucocutaneous blistering diseases characterized by acantholysis. Pemphigus has also been recognized in dogs and shares similar clinical characteristics and variants with human pemphigus. While relationships between human and canine pemphigus have been reported, gene expression patterns across species have not been described in the literature. We sought to perform gene expression analysis of lesional skin tissue from four dogs with various forms of pemphigus to examine gene expression during spontaneous disease in dogs. We found increased T and B cell signatures in canine pemphigus lesions compared to controls, as well as significant upregulation ofCCL3, CCL4, CXCL10, andCXCL8 (IL8), among other genes. Similar chemokine/cytokine expression patterns and immune infiltrates have been reported in humans, suggesting that these genes play a role in spontaneous disease. Direct comparison of our dataset to previously published human pemphigus datasets revealed five conserved differentially expressed genes:CD19, WIF1, CXCL10, CD86, andS100A12. Our data expands our understanding of pemphigus and facilitates identification of biomarkers for prediction of disease prognosis and treatment response, which may be useful for future veterinary and human clinical trials. |
| Databáze: | OpenAIRE |
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