Loss of the beta 1 subunit of the sodium pump during lymphocyte differentiation
| Autor: | Matthias Wabl, Linda Masat, Marilia Cascalho |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Gene isoform
B-Lymphocytes Protein subunit T-Lymphocytes Immunology Lymphocyte differentiation Biology Lymphocyte Activation Molecular biology Transmembrane protein TCIRG1 Mice Inbred C57BL Mice medicine.anatomical_structure medicine Immunology and Allergy Animals Bone marrow Sodium-Potassium-Exchanging ATPase Beta (finance) G alpha subunit |
| Zdroj: | European journal of immunology. 26(11) |
| ISSN: | 0014-2980 |
| Popis: | The Na,K-ATPase, or sodium pump, is responsible for maintaining cellular volume and is involved in receptor-mediated endocytosis; it is a ubiquitous transmembrane enzyme in higher eukaryotes and consists of an alpha and a beta subunit. In the mouse, two isotypes of beta with no known function have been identified: beta 1 and beta 2. We have studied the expression of beta 1 and beta 2 in lymphocytes from bone marrow, spleen, peripheral blood, and thymus. The beta 2 subunit is not expressed in any of the lymphocytes tested. Pre-B lymphocytes and the majority of mature, resting B cells in the bone marrow express the beta 1 subunit, as do all pre-T cells and mature thymocytes. In the spleen and in blood, beta 1 expression defines subsets of T and B lymphocytes. Mitogen-stimulated T and B cells lose beta 1 expression and do not express beta 2. While there is no indication that there is a change in alpha subunit isoform expression as a result of lymphocyte activation or that it is expressed in smaller amounts, there is a switch in the expression of the beta isoform. |
| Databáze: | OpenAIRE |
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