Comparison of Ophthalmic Sponges for Measurements of Immune Markers from Cervical Secretions
| Autor: | M. Concepcion Bratti, Lidia Ana Morera, Rolando Herrero, Peggy A. Crowley-Nowick, Danny Schust, Frederick P. Bowman, Allan Hildesheim, Mark Schiffman, Ana Cecilia Rodriguez, Philip E. Castle |
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| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Microbiology (medical)
Immunoglobulin A Adult Surgical Sponges Pathology medicine.medical_specialty Clinical Biochemistry Immunology Cervix Uteri Immunoglobulin G Microbiology Uterine Cervical Diseases Immune system Immunity Experimental Clinical Investigation medicine Immunology and Allergy Humans Cervix Vaccines biology Middle Aged biology.organism_classification Interleukin-12 Interleukin-10 Sponge medicine.anatomical_structure biology.protein Female Biomarkers |
| Popis: | Measurements of cervical immunity are important for evaluating immune responses to infections of the cervix and to vaccines for preventing those infections. Three ophthalmic sponges, Weck-Cel, Ultracell, and Merocel, were loaded in vitro with interleukin-1β (IL-1β), IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, IL-15, IL-18, gamma interferon (IFN-γ), granulocyte-macrophage colony-stimulating factor (GM-CSF), immunoglobulin A (IgA), or IgG, and sponges were extracted and evaluated for total recovery by enzyme-linked immunosorbent assay (ELISA). There was excellent (>75%) recovery for all immune markers from all three devices except for IL-6, which was poorly recovered ( |
| Databáze: | OpenAIRE |
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