Tyrosine hydroxylase gene expression in the locus coeruleus of depression-model rats and rats exposed to short- and long-term forced walking stress
| Autor: | Junichi Nomura, Isao Kitayama, Ping Wang |
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| Rok vydání: | 1998 |
| Předmět: |
Male
medicine.medical_specialty Tyrosine 3-Monooxygenase Tyrosine Hydroxylase Gene Spontaneous recovery Gene Expression In situ hybridization Biology General Biochemistry Genetics and Molecular Biology Physical Conditioning Animal Internal medicine Gene expression Image Processing Computer-Assisted medicine Animals Chronic stress RNA Messenger General Pharmacology Toxicology and Pharmaceutics In Situ Hybridization Messenger RNA Tyrosine hydroxylase Depression General Medicine Rats Disease Models Animal Endocrinology Locus coeruleus Locus Coeruleus Stress Psychological |
| Zdroj: | Life Sciences. 62:2083-2092 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/s0024-3205(98)00183-0 |
| Popis: | Abnormal brain noradrenergic function is thought to cause depressive illnesses which are sometimes manifested or aggravated under stressful conditions. To investigate the effect of chronic stress on noradrenaline (NA) synthesis in the brain we used in situ hybridization to examine the expression of tyrosine hydroxylase (TH) mRNA in the locus coeruleus (LC) of "depression-model rats" that exhibit reduced activity following exposure to long-term (14 days) forced walking stress (FWS). We also examined TH mRNA expression in rats stressed for 30 minutes, 3 hours and 1, 2 (short-term), 6 or 12 (long-term) days. The expression of TH mRNA increased markedly following 1 to 12 days of FWS, but not in rats exposed to FWS for 30 minutes or 3 hours. The expression also increased significantly in the depression-model rats, but not in the "spontaneous recovery rats" whose activity was restored after long-term stress. Our results suggest that NA synthesis remains high in the FWS-induced depression-model rats because of the high levels of TH mRNA expression in the LC. Our results also suggest that FWS is initially a mild stress but gradually becomes a severe form of unadaptable stress as reflected by delayed but persistent increases in TH mRNA expression. |
| Databáze: | OpenAIRE |
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